Serotyping and pathotyping of Glaesserella parasuis isolated 2012–2019 in Germany comparing different PCR-based methods

Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respiratory or Glä...

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Bibliographic Details
Published in:Veterinary research (Paris) 2020-11, Vol.51 (1), p.1-137, Article 137
Main Authors: Schuwerk, Lukas, Hoeltig, Doris, Waldmann, Karl-Heinz, Strutzberg-Minder, Katrin, Valentin-Weigand, Peter, Rohde, Judith
Format: Article
Language:English
Subjects:
Animals
Annealing
Comparative analysis
Genomes
Glaesserella parasuis
Lavage
leader sequence
Life Sciences
Medical research
Medicine, Experimental
Methods
pathotyping
Pork industry
Respiratory diseases
Respiratory tract diseases
serotyping
serovar
Software
Swine
Virulence
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Summary:Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respiratory or Glässer´s disease isolated 2012–2019 in Germany was analysed. Isolates were characterized for serovar implementing two different PCR methods. Additionally, two different PCR methods for pathotyping isolates were applied to the collection and results compared. Serovar 6 ( p  
ISSN:1297-9716
0928-4249
1297-9716
DOI:10.1186/s13567-020-00862-1