Evaluation of a Novel Synthetic Peptide Derived from Cytolytic Mycotoxin Candidalysin

• Published in: Toxins 2022-10, Vol.14 (10), p.696
• Main Authors: Cardoso, Pedro Henrique de Oliveira, Boleti, Ana Paula de Araújo, Silva, Patrícia Souza E, Mukoyama, Lincoln Takashi Hota, Guindo, Alexya Sandim, Moraes, Luiz Filipe Ramalho Nunes de, Oliveira, Caio Fernando Ramalho de, Macedo, Maria Ligia Rodrigues, Carvalho, Cristiano Marcelo Espínola, de Castro, Alinne Pereira, Migliolo, Ludovico
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Amino acids; Anti-Bacterial Agents - chemistry; Bacteria; Bacterial infections; Candida albicans; Competition; Cytokines; Cytotoxicity; drug design; E coli; Epilepsy; Escherichia coli; Evaluation; Fungi; Glial cells; Gram-negative bacteria; Immune response; Infections; Klebsiella; Macrophages; Microbial Sensitivity Tests; Microglia; Microorganisms; multiactivity peptide; Multiple sclerosis; Mycotoxins; Mycotoxins - metabolism; Mycotoxins - toxicity; neuroinflammation; Neurological diseases; Neurology; Nitrates - metabolism; peptide toxin; Peptides; Peptides - chemistry; Properties; Pseudomonas aeruginosa; Sepsis; Toxins; Tumor cell lines; Tumor cells
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins14100696

The importance of neuroinflammation in neurology is becoming increasingly apparent. In addition to neuroinflammatory diseases such as multiple sclerosis, the role of neuroinflammation has been identified in many non-inflammatory neurological disorders such as stroke, epilepsy, and cancer. The immune response within the brain involves the presence of CNS resident cells; mainly glial cells, such as microglia, the CNS resident macrophages. We evaluated the peptide Ca-MAP1 bioinspired on the immature cytolytic toxin candidalysin to develop a less hemolytic peptide with anti-neuroinflammatory, antibacterial, and cytotoxic activity against tumor cells. and studies were performed at various concentrations. Ca-MAP1 exhibits low hemolytic activity at lower concentrations and was not cytotoxic to MRC-5 and BV-2 cells. Ca-MAP1 showed activity against , ATCC, KPC, ATCC, , and ATCC. Furthermore, Ca-MAP1 exhibits anti-neuroinflammatory activity in the BV-2 microglia model, with 93.78% inhibition of nitrate production at 18.1 µM. Ca-MAP1 presents cytotoxic activity against tumor cell line NCI-H292 at 36.3 μM, with an IC of 38.4 µM. Ca-MAP1 demonstrates results that qualify it to be evaluated in the next steps to promote the control of infections and provide an alternative antitumor therapy.