Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis

is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins that contribute to host defense...

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Bibliographic Details
Published in:International journal of molecular sciences 2017-12, Vol.19 (1), p.78
Main Authors: Abdelmegid, Shaimaa, Murugaiyan, Jayaseelan, Abo-Ismail, Mohamed, Caswell, Jeff L, Kelton, David, Kirby, Gordon M
Format: Article
Language:English
Subjects:
Bioinformatics
Biomarkers
bovine
Cattle
Cell culture
Cow's milk
Mass spectrometry
Mass spectroscopy
Mastitis
Milk
milk proteins
milk proteomics
Pathogens
Proteins
Proteomics
Staphylococcus aureus
Whey
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Summary:is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins that contribute to host defense and their variable responses to pathogenic stimuli would enable the characterization of putative biomarkers of subclinical mastitis. To accomplish this, milk whey samples from healthy and mastitic dairy cows were analyzed using a label-free quantitative proteomics approach. In total, 90 proteins were identified, of which 25 showed significant differential abundance between healthy and mastitic samples. In silico functional analyses indicated the involvement of the differentially abundant proteins in biological mechanisms and signaling pathways related to host defense including pathogen-recognition, direct antimicrobial function, and the acute-phase response. This proteomics and bioinformatics analysis not only facilitates the identification of putative biomarkers of subclinical mastitis but also recapitulates previous findings demonstrating the abundance of host defense proteins in intramammary infection. All mass spectrometry data are available via ProteomeXchange with identifier .
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19010078