Diagnostic and prognostic values of miR181b-5p and miR21-5p for neonatal sepsis risk and their link to SNAP II score and disease mortality

• Published in: Non-coding RNA research 2023-03, Vol.8 (1), p.115-125
• Main Authors: Ali, Marwa A., Khamis Hussein, Sherin, Ali Mohamed, Esam, Ezzat, Mostafa Ahmed, abdelmoktader, Abdelrahman, Habib, Marwa A., Kamal, Marwa, Ahmed, Fatma A., Ali, Doaa Y.
• Format: Article
• Language: English
• Subjects: miR181b-5p; miR21-5p; Neonatal sepsis; Original; qRT-PCR; SNAP II
• ISSN: 2468-0540; 2468-0540
• DOI: 10.1016/j.ncrna.2022.11.001

Neonatal sepsis is a lethal syndrome that necessitates prompt treatment to avoid disease complications. As a result, biomarkers that may either differentiate sepsis early or predict the outcome of sepsis are essential. The goal of this research was to find out the clinical weight of using miR181b-5p and miR21-5p expression levels as diagnostic and prognostic new genetic markers for neonatal sepsis. A total of 60 neonates with sepsis and 60 healthy neonates were involved in this study. Laboratory tests include complete blood count (CBC), random blood sugar (RBS), arterial blood gases (ABG), and serum C-reactive protein (CRP). Neonates with sepsis were assessed by the Score for Neonatal Acute Physiology II (SNAP II). The serum fold changes of the target miRNAs were determined using qRT-PCR and the 2−ΔΔCt equation. The relative serum level of miR181b-5p was [ median (IQR) = 0.2509 (0.0009–4.11)] and for miR21-5p was [median (IQR) = 0.07 (0.007–7.16)] which were significantly downregulated in patients with neonatal sepsis compared to controls (p