Trypanosoma cruzi isolates from Mexican and Guatemalan acute and chronic chagasic cardiopathy patients belong to Trypanosoma cruzi I

Trypanosoma cruzi is classified into two major groups named T. cruzi I and T. cruzi II. In the present work we analyzed 16 stocks isolated from human cases and four isolated from triatomines from diverse geographical origins (Mexico and Guatemala). From human cases four were acute cases, six indeter...

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Published in:Memórias do Instituto Oswaldo Cruz 2005-05, Vol.100 (3), p.281-283
Main Authors: Ruíz-Sánchez, Rosario, León, María Paula de, Matta, Vivian, Reyes, Pedro A, López, R, Jay, David, Monteón, Victor M
Format: Article
Language:English
Subjects:
Acute Disease
Animals
Chagas Cardiomyopathy - parasitology
Chronic Disease
DNA, Protozoan - analysis
Guatemala
Humans
lineage I
Mexico
mini-exon
PARASITOLOGY
Polymerase Chain Reaction
Sequence Analysis, DNA
Triatominae - parasitology
TROPICAL MEDICINE
Trypanosoma cruzi
Trypanosoma cruzi - classification
Trypanosoma cruzi - genetics
Trypanosoma cruzi - isolation & purification
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Summary:Trypanosoma cruzi is classified into two major groups named T. cruzi I and T. cruzi II. In the present work we analyzed 16 stocks isolated from human cases and four isolated from triatomines from diverse geographical origins (Mexico and Guatemala). From human cases four were acute cases, six indeterminates, and six from chronic chagasic cardiophatic patients with diagnosis of dilated cardiomyopathy established based on the left-ventricular end systolic dimension and cardiothoracic ratio on chest X-radiography and impaired contracting ventricle and different degree conduction/rhythm aberrations. DNA samples were analyzed based on mini-exon (ME) polymorphism, using a pool of three oligonucleotide for the amplification of specific intergenic region of T. cruzi ME gene. All the Mexican and Guatemalan isolates regardless their host or vector origin generated a 350 bp amplification product. In conclusion T. cruzi I is dominant in Mexico and Guatemala even in acute and chronic chagasic cardiopathy patients. To our knowledge, this is the first study describing predominance of T. cruzi I in human infection for North and Central America.
ISSN:0074-0276
1678-8060
0074-0276
1678-8060
DOI:10.1590/S0074-02762005000300012