Multi-sensor arrays for online monitoring of cell dynamics in in vitro studies with choroid plexus epithelial cells

Sensors and multi-sensor arrays are the basis of new technologies for the non-label monitoring of cell activity. In this paper we show that choroid plexus cells can be cultured on silicon chips and that sensors register in real time changes in their activity, constituting an interesting experimental...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2012-02, Vol.12 (2), p.1383-1397
Main Authors: Mestres-Ventura, Pedro, Morguet, Andrea, de las Heras, Soledad García Gómez
Format: Article
Language:English
Subjects:
Acidification
Acidosis
Animals
Biology
Biosensing Techniques - instrumentation
cell adhesion
Cell adhesion & migration
Cell culture
cell cultures
cell respiration
Cell Separation - instrumentation
Chick Embryo
choroid plexus
Choroid Plexus - cytology
Choroid Plexus - physiology
electron microscopy
Embryos
Epithelial Cells - cytology
Epithelial Cells - physiology
Equipment Design
Equipment Failure Analysis
Flow Cytometry - instrumentation
impedance
Medical research
Metabolism
Microarray Analysis - instrumentation
Microfluidic Analytical Techniques - instrumentation
Motility
Oximetry - instrumentation
Oxygen Consumption - physiology
peri-cellular acidosis
Plethysmography, Impedance - instrumentation
Respiration
sensor arrays
Sensors
Transducers
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Summary:Sensors and multi-sensor arrays are the basis of new technologies for the non-label monitoring of cell activity. In this paper we show that choroid plexus cells can be cultured on silicon chips and that sensors register in real time changes in their activity, constituting an interesting experimental paradigm for cell biology and medical research. To validate the signals recorded (metabolism = peri-cellular acidification, oxygen consumption = respiration; impedance = adhesion, cell shape and motility) we performed experiments with compounds that act in a well-known way on cells, influencing these parameters. Our in vitro model demonstrates the advantages of multi-sensor arrays in assessment and experimental characterization of dynamic cellular events--in this case in choroid plexus functions, however with applicability to other cell types as well.
ISSN:1424-8220
1424-8220
DOI:10.3390/s120201383