Antihypertensive Effects of Corn Silk Extract and Its Novel Bioactive Constituent in Spontaneously Hypertensive Rats: The Involvement of Angiotensin-Converting Enzyme Inhibition

Corn silk tea has been used in folk medicine for anti-hypertensive healthcare. Angiotensin-converting enzyme (ACE) plays a crucial role on the homeostasis of blood pressure. However, effects of corn silk tea on ACE activity and the presence of ACE inhibitory constituents in corn silk are still unkno...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2019-05, Vol.24 (10), p.1886
Main Authors: Li, Chia-Cheng, Lee, Yu-Chen, Lo, Hsin-Yi, Huang, Yu-Wen, Hsiang, Chien-Yun, Ho, Tin-Yun
Format: Article
Language:English
Subjects:
Angiotensin
angiotensin-converting enzyme
Antihypertensives
bioactive phytopeptide
Bioinformatics
Biological activity
Blood pressure
Cardiovascular disease
Constituents
Conversion
Corn
corn silk
Diabetes
Drug dosages
Enzymes
Flavonoids
Homeostasis
Hydrogen bonding
Hydrogen bonds
Hyperglycemia
Hypertension
Oral administration
Peptides
Peptidyl-dipeptidase A
Pressure effects
Proteins
Proteomics
Silk
Tea
Vegetables
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Summary:Corn silk tea has been used in folk medicine for anti-hypertensive healthcare. Angiotensin-converting enzyme (ACE) plays a crucial role on the homeostasis of blood pressure. However, effects of corn silk tea on ACE activity and the presence of ACE inhibitory constituents in corn silk are still unknown. Here we applied proteomics and bioinformatics approaches to identify corn silk bioactive peptides (CSBps) that target ACE from the boiling water extract of corn silk (CSE). CSE significantly reduced systolic blood pressure (SBP) levels in spontaneously hypertensive rats and inhibited the ACE activity. By proteomics coupled with bioinformatics analyses, we identified a novel ACE inhibitory peptide CSBp5 in CSE. CSBp5 significantly inhibited the ACE activity and decreased SBP levels in a dose-dependent manner. Docking analysis showed that CSBp5 occupied the substrate-binding channel of ACE and interacted with ACE via hydrogen bonds. In conclusion, we identified that CSE exhibited anti-hypertensive effects in SHRs via the inhibition of ACE, the target of most anti-hypertensive drugs. In addition, an ACE inhibitory phytopeptide CSBp5 that decreased SBP levels in rats was newly identified. Our findings supported the ethnomedical use of corn silk tea on hypertension. Moreover, the identification of ACE inhibitory phytopeptide in corn silk further strengthened our findings.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24101886