Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication

Ubiquitination and deubiquitination processes are widely involved in modulating the function, activity, localization, and stability of multiple cellular proteins regulating almost every aspect of cellular function. Several virus families have been shown to exploit the cellular ubiquitin-conjugating...

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Bibliographic Details
Published in:Viruses 2023-02, Vol.15 (2), p.481
Main Authors: López, Lady S, Calvo, Eliana P, Castellanos, Jaime E
Format: Article
Language:English
Subjects:
Analysis
Antibodies
Antigens
Cell cycle
Chikungunya Fever - drug therapy
Chikungunya virus
Chikungunya virus - drug effects
deubiquitinase inhibitor
deubiquitinating enzymes
Deubiquitinating Enzymes - antagonists & inhibitors
Diagnosis
Distribution
Enzyme inhibitors
Enzyme Inhibitors - pharmacology
Enzyme Inhibitors - therapeutic use
Enzymes
Evaluation
Flow cytometry
Genomes
HEK293 Cells
Humans
infection
Infections
Localization
Membranes
Offspring
Plaque assay
Protein biosynthesis
Proteins
Replication
RNA, Viral
Transcription
Ubiquitin
Ubiquitin-proteasome system
Ubiquitination
Virus Replication - drug effects
Viruses
Western blotting
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Summary:Ubiquitination and deubiquitination processes are widely involved in modulating the function, activity, localization, and stability of multiple cellular proteins regulating almost every aspect of cellular function. Several virus families have been shown to exploit the cellular ubiquitin-conjugating system to achieve a productive infection: enter the cell, promote genome replication, or assemble and release viral progeny. In this study, we analyzed the role of deubiquitinating enzymes (DUBs) during chikungunya virus (CHIKV) infection. HEK293T, Vero-E6, and Huh-7 cells were treated with two DUB inhibitors (PR619 or WP1130). Then, infected cells were evaluated by flow cytometry, and viral progeny was quantified using the plaque assay method. The changes in viral proteins and viral RNA were analyzed using Western blotting and RT-qPCR, respectively. Results indicate that treatment with DUB inhibitors impairs CHIKV replication due to significant protein and viral RNA synthesis deregulation. Therefore, DUB activity may be a pharmacological target for blocking CHIKV infection.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15020481