Identification of key genes involved in nasopharyngeal carcinoma

• Published in: Brazilian journal of otorhinolaryngology 2017-11, Vol.83 (6), p.670-676
• Main Authors: Jiang, Xue, Feng, Lichun, Dai, Baoqiang, Li, Liping, Lu, Weiwei
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Análise de enriquecimento; Carcinoma - genetics; Carcinoma - pathology; Carcinoma nasofaríngeo; Chemokine CXCL10 - genetics; Chemokine CXCL9 - genetics; Cluster Analysis; Databases, Genetic; Differentially expressed genes; Down-Regulation; Enrichment analysis; Gene Expression; Gene Expression Profiling; Gene Regulatory Networks; Genes diferencialmente expressos; Humans; Microarray Analysis; Nasopharyngeal Carcinoma; Nasopharyngeal Neoplasms - genetics; Nasopharyngeal Neoplasms - pathology; Nuclear Proteins - genetics; Original; OTORHINOLARYNGOLOGY; Rede reguladora; Regulatory network; Transcription Factors - genetics; Up-Regulation
• ISSN: 1808-8694; 1808-8686; 1808-8686
• DOI: 10.1016/j.bjorl.2016.09.003

Nasopharyngeal carcinoma is the most common cancer originating from the nasopharynx. To study the mechanisms of nasopharyngeal carcinoma, we analyzed GSE12452 microarray data. GSE12452 was downloaded from the Gene Expression Omnibus database and included 31 nasopharyngeal carcinoma samples and 10 normal nasopharyngeal tissue samples. The differentially expressed genes were screened by ANOVA in the PGS package. Using the BiNGO plugin in Cytoscape and pathway enrichment analysis in the PGS package, functional and pathway enrichment analyses were performed separately to predict potential functions of the differentially expressed genes. Furthermore, Transcription factor-differentially expressed gene pairs were searched, and then the transcription factor-differentially expressed gene regulatory network was visualized using Cytoscape software. A total of 487 genes were screened as differentially expressed genes between the nasopharyngeal carcinoma samples and the normal nasopharyngeal tissue samples. Enrichment analysis indicated that PTGS2 was involved in the regulation of biological process and small cell lung cancer. ZIC2 and OVOL1 may function in nasopharyngeal carcinoma through targeting significantly up-regulated genes (such as PTGS2, FN1, CXCL9 and CXCL10) in the Transcription factor-differentially expressed gene regulatory network (e.g., ZIC2→PTGS2 and OVOL1→CXCL10). PTGS2, FN1, CXCL9, CXCL10, ZIC2 and OVOL1 might play roles in nasopharyngeal carcinoma. O carcinoma nasofaríngeo é o câncer mais comum originário da nasofaringe. Estudar os mecanismos do câncer de nasofaringe; dados do microarray GSE12452 foram analisados. GSE12452 foi obtido da base de dados Gene Expression Omnibus e inclui 31 amostras de carcinoma nasofaríngeo e 10 amostras de tecido nasofaríngeo normal. Os genes diferencialmente expressos foram analisados por ANOVA no kit PGS. Usando o plugin BiNGO no Cytoscape e análise de enriquecimento da via no kit PGS, análises de enriquecimento funcional e da via foram realizadas separadamente para prever as potenciais funções dos genes diferencialmente expressos. Além disso, os pares Fator de Transcrição - genes diferencialmente expressos foram pesquisados e em seguida a sua rede reguladora foi visualizada usando o programa Cytoscape. Um total de 487 genes foram analisados como genes diferencialmente expressos entre as amostras de carcinoma nasofaríngeo e amostras de tecido nasofaríngeo normal. A análise de enriquecimento indicou que PTGS2 estava e