Development of a top-down MS assay for specific identification of human periostin isoforms

Periostin is a matricellular protein encoded by the POSTN gene that is alternatively spliced to produce ten different periostin isoforms with molecular weights ranging from 78 to 91 kDa. It is known to promote fibrillogenesis, organize the extracellular matrix, and bind integrin-receptors to induce...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in molecular biosciences 2024-06, Vol.11, p.1399225
Main Authors: Rusbjerg-Weberskov, Christian E, Gant, Megan S, Chamot-Rooke, Julia, Nielsen, Nadia Sukusu, Enghild, Jan J
Format: Article
Language:English
Subjects:
Analytical chemistry
Biochemistry, Molecular Biology
chemical cleavage
Chemical Sciences
Genomics
Life Sciences
method development
Molecular Biosciences
parallel reaction monitoring
periostin
top-down mass spectrometry
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Periostin is a matricellular protein encoded by the POSTN gene that is alternatively spliced to produce ten different periostin isoforms with molecular weights ranging from 78 to 91 kDa. It is known to promote fibrillogenesis, organize the extracellular matrix, and bind integrin-receptors to induce cell signaling. As well as being a key component of the wound healing process, it is also known to participate in the pathogenesis of different diseases including atopic dermatitis, asthma, and cancer. In both health and disease, the functions of the different periostin isoforms are largely unknown. The ability to precisely determine the isoform profile of a given human sample is fundamental for characterizing their functional significance. Identification of periostin isoforms is most often carried out at the transcriptional level using RT-PCR based approaches, but due to high sequence homogeneity, identification on the protein level has always been challenging. Top-down proteomics, where whole proteins are measured by mass spectrometry, offers a fast and reliable method for isoform identification. Here we present a fully developed top-down mass spectrometry assay for the characterization of periostin splice isoforms at the protein level.
ISSN:2296-889X
2296-889X
DOI:10.3389/fmolb.2024.1399225