Detection of Fungal Diseases in Lettuce by VIR-NIR Spectroscopy in Aquaponics

• Published in: Microorganisms (Basel) 2023-09, Vol.11 (9), p.2348
• Main Authors: Sirakov, Ivaylo, Velichkova, Katya, Dinev, Toncho, Slavcheva-Sirakova, Desislava, Valkova, Elica, Yorgov, Dimitar, Veleva, Petya, Atanasov, Vasil, Atanassova, Stefka
• Format: Article
• Language: English
• Subjects: Absorption; Agricultural research; Alternaria alternata; Aquaculture; Aquaponics; Aspergillus niger; Chlorophyll; Data analysis; Diagnosis; Disease control; Disease prevention; Diseases and pests; Flowers & plants; Fruits; Fungal diseases; Fungal diseases of plants; Fungi; Fusarium oxysporum; Infections; Infrared spectra; Infrared spectroscopy; Leaves; Lettuce; Lettuces; Microorganisms; Moisture content; Near infrared radiation; Optics; pathogen detection; Pathogens; plant health; Plants (botany); prevention; Reflectance; Senescence; Spectrum analysis; Statistical analysis; Vegetables; Vegetation; Vegetation index; Water content
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11092348

One of the main challenges facing the development of aquaponics is disease control, due on one hand to the fact that plants cannot be treated with chemicals because they can lead to mortality in cultured fish. The aim of this study was to apply the visible–near-infrared spectroscopy and vegetation index approach to test aquaponically cultivated lettuce (Lactuca sativa L.) infected with different fungal pathogens (Aspergillus niger, Fusarium oxysporum, and Alternaria alternata). The lettuces on the third leaf formation were placed in tanks (with dimensions 1 m/0.50 m/0.35 m) filled up with water from the aquaponics system every second day. In this study, we included reference fungal strains Aspergillus niger NBIMCC 3252, Fusarium oxysporum NBIMCC 125, and Alternaria alternata NBIMCC 109. Diffuse reflectance spectra of the leaves of lettuce were measured directly on the plants using a USB4000 spectrometer in the 450–1100 nm wavelength range. In near-infrared spectral range, the reflectance values of infected leaves are lower than those of the control, which indicates that some changes in cell structures occurred as a result of the fungal infection. All three investigated pathogens had a statistically significant effect on leaf water content and water band index. Vegetative indices such as Chlorophyll Absorption in Reflectance Index (CARI), Modified chlorophyll absorption in reflectance index (MCARI), Plant Senescence Reflectance Index (PSRI), Red Edge Index (REI2), Red Edge Index (REI3), and Water band index (WBI) were found to be effective in distinguishing infected plants from healthy ones, with WBI demonstrating the greatest reliability.