Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding

Proteinaceous nanovaccine delivery systems have significantly promoted the development of various high-efficiency vaccines. However, the widely used method of coupling the expression of scaffolds and antigens may result in their structural interference with each other. Monovalent streptavidin (mSA)...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2022-02, Vol.12 (5), p.734
Main Authors: Shi, Yixin, Pan, Chao, Wang, Kangfeng, Liu, Yan, Sun, Yange, Guo, Yan, Sun, Peng, Wu, Jun, Lu, Ying, Zhu, Li, Wang, Hengliang
Format: Article
Language:English
Subjects:
Affinity
Antibodies
Antigens
Binding
Biotin
Cytokines
Glycosylation
Immune response
Immunization
Laboratory animals
Lymphocytes T
Modular construction
mSA
Nanoparticles
nanovaccine delivery systems
Pets
Phenols
Polysaccharides
Proteins
self-assembled proteinaceous nanoparticle
Self-assembly
Severe acute respiratory syndrome coronavirus 2
Sodium
Streptavidin
Vaccines
Vectors
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Summary:Proteinaceous nanovaccine delivery systems have significantly promoted the development of various high-efficiency vaccines. However, the widely used method of coupling the expression of scaffolds and antigens may result in their structural interference with each other. Monovalent streptavidin (mSA) is a short monomer sequence, which has a strong affinity for biotin. Here, we discuss an orthogonal, modular, and highly versatile self-assembled proteinaceous nanoparticle chassis that facilitates combinations with various antigen cargos by using mSA and biotin to produce nanovaccines. We first improved the yield of these nanoparticles by appending a short sugar chain on their surfaces in a constructed host strain. After confirming the strong ability to induce both Th1- and Th2-mediated immune responses based on the plasma cytokine spectrum from immunized mice, we further verified the binding ability of biotinylated nanoparticles to mSA-antigens. These results demonstrate that our biotinylated nanoparticle chassis could load both protein and polysaccharide antigens containing mSA at a high affinity. Our approach thus offers an attractive technology for combining nanoparticles and antigen cargos to generate various high-performance nanovaccines. In particular, the designed mSA connector (mSA containing glycosylation modification sequences) could couple with polysaccharide antigens, providing a new attractive strategy to prepare nanoscale conjugate vaccines.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano12050734