Clinical, cellular, and bioinformatic analyses reveal involvement of WRAP53 overexpression in carcinogenesis of lung adenocarcinoma

Lung cancer, of which non-small cell lung cancer accounts for 80%, remains a leading cause of cancer-related mortality and morbidity worldwide. Our study revealed that the expression of WD repeat containing antisense to P53 (WRAP53) is higher in lung-adenocarcinoma specimens than in specimens from a...

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Bibliographic Details
Published in:Tumor biology 2017-03, Vol.39 (3), p.1010428317694309-1010428317694309
Main Authors: Yuan, Xiao-Shuai, Cao, Long-Xiang, Hu, Ye-Ji, Bao, Fei-Chao, Wang, Zhi-Tian, Cao, Jin-Lin, Yuan, Ping, Lv, Wang, Hu, Jian
Format: Article
Language:English
Subjects:
A549 Cells
Adenocarcinoma
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Adenocarcinoma of Lung
Antibiotics
Antisense therapy
Authorship
Cancer therapies
Carcinogenesis
Carcinogenesis - genetics
Carcinogenesis - metabolism
Carcinogenesis - pathology
Cell culture
Cell Line, Tumor
Cell proliferation
Chemotherapy
Computational Biology
Enzymes
Female
G1 Phase Cell Cycle Checkpoints - physiology
Humans
Immunoprecipitation
Kinases
Liquid chromatography
Lung cancer
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Male
Mass spectroscopy
Medical prognosis
Medical research
Metastasis
Middle Aged
Morbidity
Non-small cell lung carcinoma
p53 Protein
Patients
Polymerase chain reaction
Proteins
S Phase - physiology
Telomerase
Telomerase - biosynthesis
Telomerase - genetics
Thoracic surgery
Tumorigenesis
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Summary:Lung cancer, of which non-small cell lung cancer accounts for 80%, remains a leading cause of cancer-related mortality and morbidity worldwide. Our study revealed that the expression of WD repeat containing antisense to P53 (WRAP53) is higher in lung-adenocarcinoma specimens than in specimens from adjacent non-tumor tissues. The prevalence of WRAP53 overexpression was significantly higher in patients with tumor larger than 3.0 cm than in patients with tumor smaller than 3.0 cm. The depletion of WRAP53 inhibits the proliferation of lung-adenocarcinoma A549 and SPC-A-1 cells via G1/S cell-cycle arrest. Several proteins interacting with WRAP53 were identified through co-immunoprecipitation and liquid chromatography/mass spectrometry. These key proteins indicated previously undiscovered functions of WRAP53. These observations strongly suggested that WRAP53 should be considered a promising target in the prevention or treatment of lung adenocarcinoma.
ISSN:1010-4283
1423-0380
DOI:10.1177/1010428317694309