Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms

The synergic effect of and increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two dif...

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Published in:Pathogens (Basel) 2023-01, Vol.12 (2), p.155
Main Authors: de Oliveira, Analú Barros, Ferrisse, Túlio Morandin, França, Gabriela Gomes, de Annunzio, Sarah Raquel, Kopp, Willian, Fontana, Carla Raquel, Brighenti, Fernanda Lourenção
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
antimicrobial photodynamic therapy
Apoptosis
Biocompatibility
Biofilms
Brazilian green propolis
Cancer
Care and treatment
cariogenic microorganisms
Color
Complications and side effects
Cytotoxicity
Dental caries
Dental materials
Diagnosis
Ethanol
Health aspects
Light
Microorganisms
natural photosensitizer
Patient outcomes
Photochemotherapy
Photodegradation
Photodynamic therapy
Properties
Propolis
Reactive oxygen species
Regrowth
Singlet oxygen
Toxicity
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Summary:The synergic effect of and increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log CFU/mL ( < 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP ( < 0.0001). BGP-mediated aPDT delayed and regrowth after 24 h of treatment ( < 0.0001). Both BGP did not change the color of dental materials ( > 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability ( < 0.0001). BGP extracts may be used in aPDT against and . Specifically, BGP-AF may represent a promising PS for dental applications.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens12020155