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Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms
The synergic effect of and increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two dif...
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| Published in: | Pathogens (Basel) 2023-01, Vol.12 (2), p.155 |
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| creator | de Oliveira, Analú Barros Ferrisse, Túlio Morandin França, Gabriela Gomes de Annunzio, Sarah Raquel Kopp, Willian Fontana, Carla Raquel Brighenti, Fernanda Lourenção |
| description | The synergic effect of
and
increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log
CFU/mL (
< 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (
< 0.0001). BGP-mediated aPDT delayed
and
regrowth after 24 h of treatment (
< 0.0001). Both BGP did not change the color of dental materials (
> 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (
< 0.0001). BGP extracts may be used in aPDT against
and
. Specifically, BGP-AF may represent a promising PS for dental applications. |
| doi_str_mv | 10.3390/pathogens12020155 |
| format | article |
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and
increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log
CFU/mL (
< 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (
< 0.0001). BGP-mediated aPDT delayed
and
regrowth after 24 h of treatment (
< 0.0001). Both BGP did not change the color of dental materials (
> 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (
< 0.0001). BGP extracts may be used in aPDT against
and
. Specifically, BGP-AF may represent a promising PS for dental applications.</description><identifier>ISSN: 2076-0817</identifier><identifier>EISSN: 2076-0817</identifier><identifier>DOI: 10.3390/pathogens12020155</identifier><identifier>PMID: 36839427</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antiinfectives and antibacterials ; antimicrobial photodynamic therapy ; Apoptosis ; Biocompatibility ; Biofilms ; Brazilian green propolis ; Cancer ; Care and treatment ; cariogenic microorganisms ; Color ; Complications and side effects ; Cytotoxicity ; Dental caries ; Dental materials ; Diagnosis ; Ethanol ; Health aspects ; Light ; Microorganisms ; natural photosensitizer ; Patient outcomes ; Photochemotherapy ; Photodegradation ; Photodynamic therapy ; Properties ; Propolis ; Reactive oxygen species ; Regrowth ; Singlet oxygen ; Toxicity</subject><ispartof>Pathogens (Basel), 2023-01, Vol.12 (2), p.155</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-d630fdb2d00ec68fca872ee5c406f757920a9b9f4efa633725e9c27d42b178fb3</citedby><cites>FETCH-LOGICAL-c560t-d630fdb2d00ec68fca872ee5c406f757920a9b9f4efa633725e9c27d42b178fb3</cites><orcidid>0000-0002-9135-3690 ; 0000-0002-4470-5171 ; 0000-0002-7177-4519 ; 0000-0002-9933-2819</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2779626723/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2779626723?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36839427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Oliveira, Analú Barros</creatorcontrib><creatorcontrib>Ferrisse, Túlio Morandin</creatorcontrib><creatorcontrib>França, Gabriela Gomes</creatorcontrib><creatorcontrib>de Annunzio, Sarah Raquel</creatorcontrib><creatorcontrib>Kopp, Willian</creatorcontrib><creatorcontrib>Fontana, Carla Raquel</creatorcontrib><creatorcontrib>Brighenti, Fernanda Lourenção</creatorcontrib><title>Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms</title><title>Pathogens (Basel)</title><addtitle>Pathogens</addtitle><description>The synergic effect of
and
increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log
CFU/mL (
< 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (
< 0.0001). BGP-mediated aPDT delayed
and
regrowth after 24 h of treatment (
< 0.0001). Both BGP did not change the color of dental materials (
> 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (
< 0.0001). BGP extracts may be used in aPDT against
and
. Specifically, BGP-AF may represent a promising PS for dental applications.</description><subject>Antiinfectives and antibacterials</subject><subject>antimicrobial photodynamic therapy</subject><subject>Apoptosis</subject><subject>Biocompatibility</subject><subject>Biofilms</subject><subject>Brazilian green propolis</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>cariogenic microorganisms</subject><subject>Color</subject><subject>Complications and side effects</subject><subject>Cytotoxicity</subject><subject>Dental caries</subject><subject>Dental materials</subject><subject>Diagnosis</subject><subject>Ethanol</subject><subject>Health aspects</subject><subject>Light</subject><subject>Microorganisms</subject><subject>natural photosensitizer</subject><subject>Patient outcomes</subject><subject>Photochemotherapy</subject><subject>Photodegradation</subject><subject>Photodynamic therapy</subject><subject>Properties</subject><subject>Propolis</subject><subject>Reactive oxygen species</subject><subject>Regrowth</subject><subject>Singlet oxygen</subject><subject>Toxicity</subject><issn>2076-0817</issn><issn>2076-0817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkt9u0zAUxiMEYtPYA3CDLHHDTYdjx3Zyg1SqMSYN6MV2bR0nx62r1C52CnSvwQvjrGOsgHNh5_j7fvL5UxQvS3rGeUPfbmBYhgX6VDLKaCnEk-KYUSUntC7V00fno-I0pRXNq6bj__PiiMuaNxVTx8XPeRjQDw56cpOQBEveR7h1vQNPLiKiJ_MYNqF3iZz_GCK0QyKQyGf8TubLMISUH-AGd4sxERsimWbW2rUxmBF5J-l2HnKIXC8xwmZHYAHOp4HMILoxgXz1aXSEuADv0jq9KJ5Z6BOe3u8nxc2H8-vZx8nVl4vL2fRq0gpJh0knObWdYR2l2MratlArhijaikqrhGoYhcY0tkILknPFBDYtU13FTKlqa_hJcbnndgFWehPdGuJOB3D6LpDfoyEOru1Rc8EVN8JYVZlKCA6GGymtYKBK7IzIrHd71mZr1ti1uaYR-gPo4Y13S70I33TTSN7IEfDmHhDD1y2mQa9darHvwWPYJs1U7p6Uisksff2XdBW20edSZZVqJMsq_ke1gJyA8zaM7RuheqoqzlnDq1F19h9V_jrMPQsercvxA0O5N-SOpRTRPuRYUj0Opv5nMLPn1ePiPDh-jyH_BT4-40M</recordid><startdate>20230117</startdate><enddate>20230117</enddate><creator>de Oliveira, Analú Barros</creator><creator>Ferrisse, Túlio Morandin</creator><creator>França, Gabriela Gomes</creator><creator>de Annunzio, Sarah Raquel</creator><creator>Kopp, Willian</creator><creator>Fontana, Carla Raquel</creator><creator>Brighenti, Fernanda Lourenção</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9135-3690</orcidid><orcidid>https://orcid.org/0000-0002-4470-5171</orcidid><orcidid>https://orcid.org/0000-0002-7177-4519</orcidid><orcidid>https://orcid.org/0000-0002-9933-2819</orcidid></search><sort><creationdate>20230117</creationdate><title>Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms</title><author>de Oliveira, Analú Barros ; Ferrisse, Túlio Morandin ; França, Gabriela Gomes ; de Annunzio, Sarah Raquel ; Kopp, Willian ; Fontana, Carla Raquel ; Brighenti, Fernanda Lourenção</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-d630fdb2d00ec68fca872ee5c406f757920a9b9f4efa633725e9c27d42b178fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antiinfectives and antibacterials</topic><topic>antimicrobial photodynamic therapy</topic><topic>Apoptosis</topic><topic>Biocompatibility</topic><topic>Biofilms</topic><topic>Brazilian green propolis</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>cariogenic microorganisms</topic><topic>Color</topic><topic>Complications and side effects</topic><topic>Cytotoxicity</topic><topic>Dental caries</topic><topic>Dental materials</topic><topic>Diagnosis</topic><topic>Ethanol</topic><topic>Health aspects</topic><topic>Light</topic><topic>Microorganisms</topic><topic>natural photosensitizer</topic><topic>Patient outcomes</topic><topic>Photochemotherapy</topic><topic>Photodegradation</topic><topic>Photodynamic therapy</topic><topic>Properties</topic><topic>Propolis</topic><topic>Reactive oxygen species</topic><topic>Regrowth</topic><topic>Singlet oxygen</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira, Analú Barros</creatorcontrib><creatorcontrib>Ferrisse, Túlio Morandin</creatorcontrib><creatorcontrib>França, Gabriela Gomes</creatorcontrib><creatorcontrib>de Annunzio, Sarah Raquel</creatorcontrib><creatorcontrib>Kopp, Willian</creatorcontrib><creatorcontrib>Fontana, Carla Raquel</creatorcontrib><creatorcontrib>Brighenti, Fernanda Lourenção</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals(OpenAccess)</collection><jtitle>Pathogens (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Oliveira, Analú Barros</au><au>Ferrisse, Túlio Morandin</au><au>França, Gabriela Gomes</au><au>de Annunzio, Sarah Raquel</au><au>Kopp, Willian</au><au>Fontana, Carla Raquel</au><au>Brighenti, Fernanda Lourenção</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms</atitle><jtitle>Pathogens (Basel)</jtitle><addtitle>Pathogens</addtitle><date>2023-01-17</date><risdate>2023</risdate><volume>12</volume><issue>2</issue><spage>155</spage><pages>155-</pages><issn>2076-0817</issn><eissn>2076-0817</eissn><abstract>The synergic effect of
and
increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log
CFU/mL (
< 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (
< 0.0001). BGP-mediated aPDT delayed
and
regrowth after 24 h of treatment (
< 0.0001). Both BGP did not change the color of dental materials (
> 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (
< 0.0001). BGP extracts may be used in aPDT against
and
. Specifically, BGP-AF may represent a promising PS for dental applications.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36839427</pmid><doi>10.3390/pathogens12020155</doi><orcidid>https://orcid.org/0000-0002-9135-3690</orcidid><orcidid>https://orcid.org/0000-0002-4470-5171</orcidid><orcidid>https://orcid.org/0000-0002-7177-4519</orcidid><orcidid>https://orcid.org/0000-0002-9933-2819</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Pathogens (Basel), 2023-01, Vol.12 (2), p.155 |
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| language | eng |
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| source | Publicly Available Content Database; PubMed Central(OpenAccess) |
| subjects | Antiinfectives and antibacterials antimicrobial photodynamic therapy Apoptosis Biocompatibility Biofilms Brazilian green propolis Cancer Care and treatment cariogenic microorganisms Color Complications and side effects Cytotoxicity Dental caries Dental materials Diagnosis Ethanol Health aspects Light Microorganisms natural photosensitizer Patient outcomes Photochemotherapy Photodegradation Photodynamic therapy Properties Propolis Reactive oxygen species Regrowth Singlet oxygen Toxicity |
| title | Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms |
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