Microsatellite Loci for Orthophytum ophiuroides (Bromelioideae, Bromeliaceae) Species Adapted to Neotropical Rock Outcrops

Premise of the study: Microsatellite primers were developed for Orthophytum ophiuroides, a rupicolous bromeliad species endemic to neotropical rocky fields. These microsatellite loci will be used to investigate population differentiation and species cohesion in such fragmented environments. The loci...

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Bibliographic Details
Published in:Applications in plant sciences 2014-03, Vol.2 (3), p.1300073-n/a
Main Authors: Aoki-Gonçalves, Felipe, Louzada, Rafael B, Souza, Lívia Moura De, Palma-Silva, Clarisse
Format: Article
Language:English
Subjects:
alleles
Bromeliaceae
Cloning
cohesion
cross-amplification
Deoxyribonucleic acid
DNA
Endemic species
Gene flow
Genetic diversity
Genetic markers
genetic variation
habitats
indigenous species
loci
microsatellite repeats
Microsatellites
Orthophytum ophiuroides
Plant sciences
Population
Population differentiation
Population genetics
PRIMER NOTES
tropics
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Summary:Premise of the study: Microsatellite primers were developed for Orthophytum ophiuroides, a rupicolous bromeliad species endemic to neotropical rocky fields. These microsatellite loci will be used to investigate population differentiation and species cohesion in such fragmented environments. The loci were tested for cross-amplification in related bromeliad species. Methods and Results: Eleven polymorphic microsatellite markers were isolated and characterized from an enriched library of O. ophiuroides. The loci were tested on 42 individuals from two populations of this species. The number of alleles per locus ranged from three to nine and the expected and observed heterozygosities ranged from 0.167 to 0.870 and from 0.369 to 0.958, respectively. Seven loci successfully amplified in other related bromeliad species. Conclusions: Our results suggest that the microsatellite loci developed here will be useful to assess genetic diversity and gene flow in O. ophiuroides for the investigation of population differentiation and species cohesion in neotropical mountainous habitats.
ISSN:2168-0450
2168-0450
DOI:10.3732/apps.1300073