Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test

Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, incl...

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Published in:Clinical and Biomedical Research 2014-07, Vol.34 (2)
Main Authors: Franciéli Pedrotti Rozales, Fernada de-Paris, Alice Beatriz Mombach Pinheiro Machado, Cintia Costi, Afonso Luis Barth
Format: Article
Language:English
Subjects:
Acute disease
Diagnostic test
HCV
X-tail
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container_issue 2
container_start_page
container_title Clinical and Biomedical Research
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creator Franciéli Pedrotti Rozales
Fernada de-Paris
Alice Beatriz Mombach Pinheiro Machado
Cintia Costi
Afonso Luis Barth
description Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure.
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ispartof Clinical and Biomedical Research, 2014-07, Vol.34 (2)
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subjects Acute disease
Diagnostic test
HCV
X-tail
title Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
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