Intestinal response of Rana chensinensis larvae exposed to Cr and Pb, alone and in combination

• Published in: Ecotoxicology and environmental safety 2023-04, Vol.255, p.114774-114774, Article 114774
• Main Authors: Zhang, Siliang, Chen, Aixia, Deng, Hongzhang, Jiang, Ling, Liu, Xiaoli, Chai, Lihong
• Format: Article
• Language: English
• Subjects: Amphibians; Animals; Digestive enzyme; Gastrointestinal Microbiome; Gut microbiome; Heavy metal; Intestines; Larva; Lead - toxicity; Ranidae - genetics; Transcriptional expression; Verrucomicrobia
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2023.114774

Although numerous investigations on the adverse impact of Cr and Pb have been performed, studies on intestinal homeostasis in amphibians are limited. Here, single and combined effects of Cr (104 μg/L) and Pb (50 μg/L) on morphological and histological features, bacterial community, digestive enzymes activities, as well as transcriptomic profile of intestines in Rana chensinensis tadpoles were assessed. Significant decrease in the relative intestine length (intestine length/snout-to-vent length, IL/SVL) was observed after exposure to Pb and Cr/Pb mixture. Intestinal histology and digestive enzymes activities were altered in metal treatment groups. In addition, treatment groups showed significantly increased bacterial richness and diversity. Tadpoles in treatment groups were observed to have differential gut bacterial composition from controls, especially for the abundance of phylum Proteobacteria, Firmicutes, Verrucomicrobia, Actinobacteria, and Fusobacteria as well as genus Citrobacter, Anaerotruncus, Akkermansia, and Alpinimonas. Moreover, transcriptomic analysis showed that the transcript expression profiles of GPx and SOD isoforms responded differently to Cr and/or Pb exposure. Besides, transcriptional activation of pro-apoptotic and glycolysis-related genes, such as Bax, Apaf 1, Caspase 3, PK, PGK, TPI, and GPI were detected in all treatment groups but downregulation of Bcl2 in Pb and Cr/Pb mixture groups. Collectively, these results suggested that Cr and Pb exposure at environmental relevant concentration, alone and in combination, could disrupt intestinal homeostasis of R. chensinensis tadpoles. [Display omitted] •Cr and/or Pb exposure caused histological damage in intestine.•Cr and/or Pb exposure altered gut bacterial community diversity and structure.•More DEGs were detected in Cr/Pb mixture group, as compared with Cr and Pb exposure alone.•Cr and/or Pb exposure enhanced transcriptional response of pro-apoptotic and glycolysis-related genes.