The Lipid Raft Component Stomatin Interacts with the Na + Taurocholate Cotransporting Polypeptide (NTCP) and Modulates Bile Salt Uptake

• Published in: Cells (Basel, Switzerland) Switzerland), 2020-04, Vol.9 (4), p.986
• Main Authors: Appelman, Monique D, Robin, Marion J D, Vogels, Esther W M, Wolzak, Christie, Vos, Winnie G, Vos, Harmjan R, Van Es, Robert M, Burgering, Boudewijn M T, Van de Graaf, Stan F J
• Format: Article
• Language: English
• Subjects: ASBT; bile acid; Bile Acids and Salts - metabolism; Biological Transport, Active - genetics; BSEP; Cell Line, Tumor; Cell Membrane - metabolism; Chloride Channels - genetics; Chloride Channels - metabolism; cholestasis; Chromatography, Liquid; enterohepatic circulation; Gene Knockdown Techniques; Hepatocytes - metabolism; Humans; lipid raft; Liver - metabolism; Membrane Microdomains - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Organic Anion Transporters, Sodium-Dependent - genetics; Organic Anion Transporters, Sodium-Dependent - metabolism; Protein Binding; Symporters - genetics; Symporters - metabolism; Tandem Mass Spectrometry; Taurocholic Acid - metabolism
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells9040986

The sodium taurocholate cotransporting polypeptide (NTCP) is expressed at the basolateral membrane of hepatocytes, where it mediates the uptake of conjugated bile acids and forms the hepatocyte entry receptor for the hepatitis B and D virus. Here, we aimed to identify novel protein-protein interactions that could play a role in the regulation of NTCP. To this end, NTCP was precipitated from HA-tagged hNTCP-expressing HepG2 cells, and chloride channel CLIC-like 1 (CLCC1) and stomatin were identified as interacting proteins by mass spectrometry. Interaction was confirmed by co-immunoprecipitation. NTCP, CLCC1 and stomatin were found at the plasma membrane in lipid rafts, as demonstrated by a combination of immunofluorescence, cell surface biotinylation and isolation of detergent-resistant membranes. Neither CLCC1 overexpression nor its knockdown had an effect on NTCP function. However, both stomatin overexpression and knockdown increased NTCP-mediated taurocholate uptake while NTCP abundance at the plasma membrane was only increased in stomatin depleted cells. These findings identify stomatin as an interactor of NTCP and show that the interaction modulates bile salt transport.