The role of TAp63γ and P53 point mutations in regulating DNA repair, mutational susceptibility and invasion of bladder cancer cells

• Published in: eLife 2021-11, Vol.10
• Main Authors: Wang, Hsiang-Tsui, Lee, Hyun-Wook, Weng, Mao-wen, Liu, Yan, Huang, William C, Lepor, Herbert, Wu, Xue-Ru, Tang, Moon-shong
• Format: Article
• Language: English
• Subjects: Bladder cancer; Cancer Biology; Deoxyribonucleic acid; DNA; DNA damage; DNA repair; Enzymes; Fibroblasts; Genes; Invasiveness; Mutagenesis; Mutation; mutation susceptibility; p53 Protein; p53m; Phenotypes; Plasmids; Susceptibility; TAp63g; tumor invasion; Tumor suppressor genes; Tumors; XPC protein
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.71184

It has long been recognized that non-muscle-invasive bladder cancer (NMIBC) has a low propensity (20%) of becoming muscle-invasive (MIBC), and that MIBC carry many more p53 point mutations (p53m) than NMIBC (50% vs 10%). MIBC also has a higher mutation burden than NMIBC. These results suggest that DNA repair capacities, mutational susceptibility and p53m are crucial for MIBC development. We found MIBC cells are hypermutable, deficient in DNA repair and have markedly downregulated DNA repair genes, XPC, hOGG1/2 and Ref1, and the tumor suppressor, TAp63γ. In contrast, NMIBC cells are hyperactive in DNA repair and exhibit upregulated DNA repair genes and TAp63γ. A parallel exists in human tumors, as MIBC tissues have markedly lower DNA repair activity, and lower expression of DNA repair genes and TAp63γ compared to NMIBC tissues. Forced TAp63γ expression in MIBC significantly mitigates DNA repair deficiencies and reduces mutational susceptibility. Knockdown of TAp63γ in NMIBC greatly reduces DNA repair capacity and enhances mutational susceptibility. Manipulated TAp63γ expression or knockdown of p53m reduce the invasion of MIBC by 40–60%. However, the combination of p53m knockdown with forced TAp63γ expression reduce the invasion ability to nil suggesting that p53m contributes to invasion phenotype independent from TAp63γ. These results indicate that in BC, TAp63γ regulates DNA repair capacities, mutational susceptibility and invasion, and that p53m contribute to the invasion phenotype. We conclude that concurrent TAp63γ suppression and acquisition of p53m are a major cause for MIBC development.