Bacteriophage Infection of the Marine Bacterium Shewanella glacialimarina Induces Dynamic Changes in tRNA Modifications

Viruses are obligate intracellular parasites that, throughout evolution, have adapted numerous strategies to control the translation machinery, including the modulation of post-transcriptional modifications (PTMs) on transfer RNA (tRNA). PTMs are critical translation regulators used to further host...

Full description

Saved in:
Bibliographic Details
Published in:Microorganisms (Basel) 2023-01, Vol.11 (2), p.355
Main Authors: Lampi, Mirka, Gregorova, Pavlina, Qasim, M Suleman, Ahlblad, Niklas C V, Sarin, L Peter
Format: Article
Language:English
Subjects:
Adsorption
Anticodons
Bacteria
Genomes
HIV
host–pathogen interaction
Human immunodeficiency virus
Immune response
Infections
Parasites
Phages
Polyethylene glycol
Post-transcription
post-transcriptional nucleoside modification
Queuosine
Regulatory mechanisms (biology)
Shewanella
Shewanella glacialimarina
Shewanella phage 1/4
Transfer RNA
Translation
tRNA
tRNA Ala
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Viruses are obligate intracellular parasites that, throughout evolution, have adapted numerous strategies to control the translation machinery, including the modulation of post-transcriptional modifications (PTMs) on transfer RNA (tRNA). PTMs are critical translation regulators used to further host immune responses as well as the expression of viral proteins. Yet, we lack critical insight into the temporal dynamics of infection-induced changes to the tRNA modification landscape (i.e., 'modificome'). In this study, we provide the first comprehensive quantitative characterization of the tRNA modificome in the marine bacterium during Shewanella phage 1/4 infection. Specifically, we show that PTMs can be grouped into distinct categories based on modification level changes at various infection stages. Furthermore, we observe a preference for the UAC codon in viral transcripts expressed at the late stage of infection, which coincides with an increase in queuosine modification. Queuosine appears exclusively on tRNAs with GUN anticodons, suggesting a correlation between phage codon usage and PTM modification. Importantly, this work provides the basis for further studies into RNA-based regulatory mechanisms employed by bacteriophages to control the prokaryotic translation machinery.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11020355