Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes ( ELPs ) Gene Family of Wheat: TaELPs Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence

• Published in: Plants (Basel) 2023-02, Vol.12 (4), p.952
• Main Authors: Guo, Feng, Islam, Md Ashraful, Lv, Chenxu, Jin, Xiujuan, Sun, Lili, Zhao, Kai, Lu, Juan, Yan, Rongyue, Zhang, Wenjun, Shi, Yugang, Li, Ning, Sun, Daizhen
• Format: Article
• Language: English
• Subjects: Abiotic stress; Acetylation; Amino acids; Bioinformatics; Chromosomes; Cluster analysis; Defense mechanisms; DNA-directed RNA polymerase; Drought; Elongation; Elongator complexes (ELPs); Gene duplication; Gene expression; Genes; Genomes; Histones; Homology; in silico analysis; Kinases; leaf senescence; Leaves; Localization; Oxidative stress; Pathogens; Phylogeny; Physiology; Plant growth; Plant resistance; Plant species; Plants (botany); Proteins; Regulatory mechanisms (biology); Regulatory sequences; Reproduction (copying); RNA polymerase; RNA polymerase II; Senescence; Signal transduction; Stresses; Transcription; Transcription factors; Transfer RNA; Wheat
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12040952

Elongator complexes ( ) are the protein complexes that promote transcription through histone acetylation in eukaryotic cells and interact with elongating RNA polymerase II (RNAPII). ' role in plant growth and development, signal transduction, and response to biotic and abiotic stresses have been confirmed in model plants. However, the functions of the wheat genes are not well documented. The present study identified 18 members of the from the wheat genome with a homology search. Further, bioinformatics and transcription patterns in response to different stress conditions were analyzed to dissect their potential regulatory mechanisms in wheat. Gene duplication analysis showed that 18 pairs of paralogous genes were derived from segmental duplication, which was divided into six clades by protein phylogenetic and cluster analysis. The orthologous analysis of wheat genes showed that genes may have evolved from orthologous genes of other plant species or closely related plants. Moreover, a variety of cis-acting regulatory elements (CAREs) related to growth and development, hormone response, and biotic and abiotic stresses were identified in the promoter regions. The qRT-PCR analysis showed that the transcription of was induced under hormone, salt, and drought stress and during leaf senescence. The gene was silenced with BSMV-VIGS, and was preliminarily verified to be involved in the regulation of wheat leaf senescence. Overall, genes might be regulated by hormone signaling pathways and response to abiotic stress and leaf senescence, which could be investigated further as potential candidate genes for wheat abiotic stress tolerance and yield improvement.