Utility of bronchoscopically obtained frozen cytology pellets for next-generation sequencing

Next-generation sequencing (NGS) is essential for lung cancer treatment. It is important to collect sufficient tissue specimens, but sometimes we cannot obtain large enough samples for NGS analysis. We investigated the yield of NGS analysis by frozen cytology pellets using an Oncomine Comprehensive...

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Bibliographic Details
Published in:BMC cancer 2024-04, Vol.24 (1), p.489-489, Article 489
Main Authors: Mimura, Chihiro, Takamiya, Rei, Fujimoto, Shodai, Fukui, Takafumi, Yatani, Atsuhiko, Yamada, Jun, Takayasu, Mizuki, Takata, Naoya, Sato, Hiroki, Fukuda, Kiyoko, Furukawa, Koichi, Hazama, Daisuke, Katsurada, Naoko, Yamamoto, Masatsugu, Matsumoto, Shingo, Goto, Koichi, Tachihara, Motoko
Format: Article
Language:English
Subjects:
Analysis
Bronchoscopy
Care and treatment
Cytology
DNA sequencing
Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA)
Evaluation
Lung cancer
Next-generation sequencing (NGS)
Nucleotide sequencing
Patient outcomes
Pellet specimen
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Summary:Next-generation sequencing (NGS) is essential for lung cancer treatment. It is important to collect sufficient tissue specimens, but sometimes we cannot obtain large enough samples for NGS analysis. We investigated the yield of NGS analysis by frozen cytology pellets using an Oncomine Comprehensive Assay or Oncomine Precision Assay. We retrospectively enrolled patients with lung cancer who underwent bronchoscopy at Kobe University Hospital and were enrolled in the Lung Cancer Genomic Screening Project for Individualized Medicine. We investigated the amount of extracted DNA and RNA and determined the NGS success rates. We also compared the amount of DNA and RNA by bronchoscopy methods. To create the frozen cytology pellets, we first effectively collected the cells and then quickly centrifuged and cryopreserved them. A total of 132 patients were enrolled in this study between May 2016 and December 2022; of them, 75 were subjected to frozen cytology pellet examinations and 57 were subjected to frozen tissue examinations. The amount of DNA and RNA obtained by frozen cytology pellets was nearly equivalent to frozen tissues. Frozen cytology pellets collected by endobronchial ultrasound-guided transbronchial needle aspiration yielded significantly more DNA than those collected by transbronchial biopsy methods. (P 
ISSN:1471-2407
1471-2407
DOI:10.1186/s12885-024-12250-5