Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse

Food is often contaminated with Escherichia coli (E. coli) bacteria strains, which have been associated with different diseases, including urinary tract infections. The consumption of meat by humans is a potential route of transmission of antimicrobial resistance, and food-producing animals have bee...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-08, Vol.11 (8), p.2065
Main Authors: Herrera-Vázquez, Arturo, Arellano-Aranda, Rebeca, Hernández-Cueto, Daniel, Rodríguez-Miranda, Esmeralda, López-Briones, Sergio, Hernández-Luna, Marco Antonio
Format: Article
Language:English
Subjects:
Abattoirs
Antibiotics
Antimicrobial resistance
Bacteria
CNF-1
Coliforms
Drug resistance
E coli
Escherichia coli
Food consumption
Food contamination
Food contamination & poisoning
Foodborne pathogens
Genes
Health care
Hogs
Illnesses
Inflammation
Kidneys
Meat products
Microorganisms
pig kidney
Polymerase chain reaction
Pork
Proteins
Strains (organisms)
Swine
Toxins
Urinary tract
Urinary tract diseases
urinary tract infection
Urinary tract infections
Urogenital system
Virulence
Western blotting
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Summary:Food is often contaminated with Escherichia coli (E. coli) bacteria strains, which have been associated with different diseases, including urinary tract infections. The consumption of meat by humans is a potential route of transmission of antimicrobial resistance, and food-producing animals have been associated as a major reservoir of resistant bacterial strains. The aim of this study was to determine the presence of the E. coli strains producing the CNF-1 toxin in pig kidneys. Pig kidneys were collected from a Mexican slaughterhouse and classified according to their coloration into reddish kidneys (RK) and yellowish kidneys (YK). A tissue sample from each kidney was processed for histological analysis, the presence of E. coli was determined by conventional PCR assay, and the CNF-1 toxin was detected by both conventional PCR and Western blotting. Herein, an inflammatory cell infiltrate was found in all collected kidneys, regardless of macroscopic differences. Surprisingly, E. coli and the CNF-1 toxin were detected in all kidney samples. We clearly demonstrate contamination by CNF-1 toxin-producing E. coli in pork kidneys from a slaughterhouse, even in those without apparent damage. This suggests that pork may serve as a reservoir for pathogens, representing an important risk to human health.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11082065