Experimental study of an evanescent-field biosensor based on 1D photonic bandgap structures

A photonic bandgap (PBG) biosensor has been developed for the label-free detection of proteins. As the sensing in this type of structures is governed by the interaction between the evanescent field going into the cladding and the target analytes, scanning near-field optical microscopy has been used...

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Bibliographic Details
Published in:Beilstein journal of nanotechnology 2019, Vol.10 (1), p.967-974
Main Authors: Sabek, Jad, Díaz-Fernández, Francisco Javier, Torrijos-Morán, Luis, Díaz-Betancor, Zeneida, Maquieira, Ángel, Bañuls, María-José, Pinilla-Cienfuegos, Elena, García-Rupérez, Jaime
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Binding sites
Biosensors
Cladding
evanescent field
Full Research Paper
half-antibodies
Immunoglobulins
Light
light-assisted immobilization
Microscopy
Nanoscience
Nanotechnology
Optical microscopy
Photonic band gaps
photonic bandgap sensor
Photonics
Semiconductors
Sensors
Serum albumin
SNOM characterization
Target detection
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Summary:A photonic bandgap (PBG) biosensor has been developed for the label-free detection of proteins. As the sensing in this type of structures is governed by the interaction between the evanescent field going into the cladding and the target analytes, scanning near-field optical microscopy has been used to characterize the profile of that evanescent field. The study confirms the strong exponential decrease of the signal as it goes into the cladding. This means that biorecognition events must occur as close to the PBG structure surface as possible in order to obtain the maximum sensing response. Within this context, the PBG biosensor has been biofunctionalized with half-antibodies specific to bovine serum albumin (BSA) using a UV-induced immobilization procedure. The use of half-antibodies allows one to reduce the thickness of the biorecognition volume down to ca. 2.5 nm, thus leading to a higher interaction with the evanescent field, as well as a proper orientation of their binding sites towards the target sample. Then, the biofunctionalized PBG biosensor has been used to perform a direct and real-time detection of the target BSA antigen.
ISSN:2190-4286
2190-4286
DOI:10.3762/bjnano.10.97