First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain

Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequ...

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Bibliographic Details
Published in:Plants (Basel) 2021-10, Vol.10 (11), p.2293
Main Authors: Canales, Celia, Morán, Félix, Olmos, Antonio, Ruiz-García, Ana Belén
Format: Article
Language:English
Subjects:
ACLSV
AHVd
Apples
ASGV
Biodiversity
Crop diseases
CTLV
Cultivars
Fruits
Genomes
Grooving
HTS
Leafspot
Leaves
loquat
Next-generation sequencing
Orchards
Phylogenetics
Phylogeny
Plant virus diseases
Plant viruses
Polymerase chain reaction
Proteins
RNA polymerase
Sequences
Stem pitting
Stems
Viroids
Viruses
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Summary:Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10112293