Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors

The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from t...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2017-06, Vol.17 (7), p.1506
Main Authors: Schuller, Astrid, Rödel, Gerhard, Ostermann, Kai
Format: Article
Language:English
Subjects:
ATP-Binding Cassette Transporters
Biosensing Techniques
biosensor
Biosensors
Diclofenac
Fluorescence
Nonsteroidal anti-inflammatory drugs
Organic chemistry
PDR5
Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Sensitivity analysis
Yeast
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Summary:The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from the authentic promoter of the gene. This key component of the pleiotropic drug response encodes a multidrug transporter that is involved in cellular detoxification. We analyse the effects on diclofenac sensitivity of artificial promoter derivatives in wild-type and various yeast mutant strains. This approach enabled us to generate sensor strains with elevated drug sensitivity.
ISSN:1424-8220
1424-8220
DOI:10.3390/s17071506