In Vitro and In Vivo Photoprotective Effects of (-)-Loliode Isolated from the Brown Seaweed, Sargassum horneri

• Published in: Molecules (Basel, Switzerland) Switzerland), 2021-11, Vol.26 (22), p.6898
• Main Authors: Wang, Lei, Kim, Hyun-Soo, Je, Jun-Geon, Fu, Xiaoting, Huang, Caoxing, Ahn, Ginnae, Oh, Jae-Young, Sanjeewa, K K Asanka, Xu, Jiachao, Gao, Xin, Yeo, In-Kyu, Jeon, You-Jin
• Format: Article
• Language: English
• Subjects: (-)-Loliode; Algae; Animals; Antioxidants; Apoptosis; Apoptosis - drug effects; Apoptosis - radiation effects; Benzofurans - chemistry; Benzofurans - isolation & purification; Benzofurans - pharmacology; Biomedical materials; Cell death; Cell Line; Cell viability; Collagen; Cosmeceuticals; Cosmetics; Cytotoxicity; Danio rerio; Dermis - metabolism; Environmental factors; Fibroblasts - metabolism; Humans; In vivo methods and tests; Inflammation; Keratinocytes; Keratinocytes - metabolism; Lipid peroxidation; Lipids; Matrix metalloproteinase; Matrix metalloproteinases; MMPs; Nitric oxide; Oxidative stress; Peroxidation; Radiation damage; Reactive oxygen species; Reactive Oxygen Species - metabolism; ROS; Sargassum - chemistry; Sargassum horneri; Seaweed - chemistry; Seaweeds; Skin; Sunscreening Agents - chemistry; Sunscreening Agents - isolation & purification; Sunscreening Agents - pharmacology; Ultraviolet Rays - adverse effects; UVB irradiation; Zebrafish; Zebrafish - metabolism
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26226898

Skin is the largest organ of humans. Overexposure to ultraviolet (UV) is the primary environmental factor that causes skin damage. The compound, (-)-loliode, isolated from the brown seaweed , showed strong antioxidant and anti-inflammatory activities in in vitro and in vivo models. To further explore the potential of (-)-loliode in cosmetics, in the present study, we investigated the photoprotective effect of (-)-loliode in vitro in skin cells and in vivo in zebrafish. The results indicated that (-)-loliode significantly reduced intracellular reactive oxygen species (ROS) level, improved cell viability, and suppressed apoptosis of UVB-irradiated human keratinocytes. In addition, (-)-loliode remarkably attenuated oxidative damage, improved collagen synthesis, and inhibited matrix metalloproteinases expression in UVB-irradiated human dermal fibroblasts. Furthermore, the in vivo test demonstrated that (-)-loliode effectively and dose-dependently suppressed UVB-induced zebrafish damage displayed in decreasing the levels of ROS, nitric oxide, lipid peroxidation, and cell death in UVB-irradiated zebrafish. These results indicate that (-)-loliode possesses strong photoprotective activities and suggest (-)-loliode may an ideal ingredient in the pharmaceutical and cosmeceutical industries.