The detection and utilization of volatile metabolomics in Klebsiella pneumoniae by gas chromatography-ion mobility spectrometry

• Published in: Scientific reports 2024-10, Vol.14 (1), p.26122-16, Article 26122
• Main Authors: Li, Fuxing, Gu, Shumin, Zhao, Chuwen, Zheng, Yunwei, Zhu, Junqi, Hu, Longhua, Hang, Yaping
• Format: Article
• Language: English
• Subjects: 631/326/107; 631/326/2521; 631/326/2522; Bacterial Proteins - metabolism; beta-Lactamases - metabolism; Carbapenemase; Gas chromatography-ion mobility spectrometry (GC-IMS); Gas Chromatography-Mass Spectrometry - methods; Humanities and Social Sciences; Humans; Imipenem (IPM); Imipenem - pharmacology; Ion Mobility Spectrometry - methods; Klebsiella pneumoniae; Klebsiella pneumoniae - metabolism; Metabolomics - methods; multidisciplinary; Science; Science (multidisciplinary); Volatile metabolomic; Volatile Organic Compounds - analysis; Volatile Organic Compounds - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-77746-3

This research aimed to analyze the volatile compounds emitted during the proliferation of Klebsiella pneumoniae ( K. pneumoniae ) in the laboratory setting using gas chromatography-ion mobility spectrometry (GC-IMS) and to investigate the potential of volatile metabolomics for detecting carbapenemase-producing strains of K. pneumoniae . The volatile metabolomics of K. pneumoniae were comprehensively analyzed using GC-IMS in tryptic soy broth (TSB) as the culture medium. Afterward, the growth stabilization period (T2) served as the primary time point for analysis, with the introduction of imipenem and carbapenemase inhibitors (avibactam sodium or EDTA) during the exponential growth phase (T0) to further investigate alterations in volatile molecules associated with K. pneumoniae . Standard strains were utilized as references, while clinical strains were employed for validation purposes. At T2, a total of 22 volatile organic compounds (VOCs) associated with K. pneumoniae were identified (3 VOCs found in both monomer and dimer forms). Significant differences in VOCs were observed between carbapenemase-negative and carbapenemase-positive strains, both standard and clinical, following the introduction of imipenem. Furthermore, the addition of avibactam sodium led to distinct changes in the VOC content of strains producing class A carbapenemase, while the addition of EDTA resulted in specific alterations in the volatile metabolic profiles of strains producing class B carbapenemase. GC-IMS demonstrated significant promise for analyzing bacterial volatile metabolomics, and its application in evaluating the volatolomics of K. pneumoniae may facilitate the timely detection of carbapenemase-producing strains.