Construction of gene modification system with highly efficient and markerless for Monascus ruber M7

Monascus spp. are traditional medicinal and edible filamentous fungi in China, and can produce various secondary metabolites, such as Monascus pigments (MPs) and citrinin (CIT). Genetic modification methods, such as gene knock-out, complementation, and overexpression, have been used extensively to i...

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Bibliographic Details
Published in:Frontiers in microbiology 2022-08, Vol.13, p.952323-952323
Main Authors: Xu, Na, Li, Li, Chen, Fusheng
Format: Article
Language:English
Subjects:
genetic modification system
Microbiology
Monascus ruber M7
mrlig4
mrpyrG
resistance selection marker
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Summary:Monascus spp. are traditional medicinal and edible filamentous fungi in China, and can produce various secondary metabolites, such as Monascus pigments (MPs) and citrinin (CIT). Genetic modification methods, such as gene knock-out, complementation, and overexpression, have been used extensively to investigate the function of related genes in Monascus spp.. However, the resistance selection genes that can have been used for genetic modification in Monascus spp. are limited, and the gene replacement frequency (GRF) is usually
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2022.952323