Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential

belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities) of the isolates...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-02, Vol.24 (4), p.741
Main Authors: Blažević, Ivica, Đulović, Azra, Čikeš Čulić, Vedrana, Burčul, Franko, Ljubenkov, Ivica, Ruščić, Mirko, Generalić Mekinić, Ivana
Format: Article
Language:English
Subjects:
anti-oxidation
Ascorbic acid
Bladder cancer
Bunias erucago L
Carotene
cholinesterase inhibition
cytotoxic activity
Cytotoxicity
desulfoglucosinolates
Distillates
Fibers
Free radicals
glucosinolates
isothiocyanates
Methods
Minerals
nitriles
Oils & fats
Polyphenols
Scavenging
Sulfur
Tocopherol
β-Carotene
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Summary:belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities) of the isolates containing glucosinolate breakdown products. The chemical profiles were determined by using HPLC-PDA-MS/MS of desulfoglucosinolates and GC-MS of glucosinolate degradation products. The analysis of showed the presence of seven glucosinolates: gluconapin ( ), glucoraphasatin ( ), glucoraphenin ( ), glucoerucin ( ), glucoraphanin ( ), glucotropaeolin ( ), and glucosinalbin ( ). The total glucosinolate content ranged from 7.0 to 14.6 µmol/g of dry weight, with the major glucosinolate glucosinalbin in all parts. The antioxidant activity of all volatile isolates was not notable. At a tested concentration of 227 μg/mL, flower hydro-distillate (FH) showed good AChE inhibition, i.e., 40.9%, while root hydro-distillate (RH) had good activity against BChE, i.e., 54.3%. FH showed the best activity against both tested human bladder cancer cell lines, i.e., against T24 after 72 h, which have IC of 16.0 μg/mL, and against TCCSUP after 48 h with IC of 7.8 μg/mL, and can be considered as highly active. On the other hand, RH showed weak activity against tested cancer cells.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24040741