Noradrenaline increases intracellular Ca2+ concentration in epithelial cells via α2-adrenoceptors in isolated mouse ileal crypts

The stimulation of α2-adrenoceptors caused a transient increase of intracellular calcium concentration ([Ca2+]i) monitored by ratiometry using Fura-2 in epithelial cells including enterochromaffin cells in isolated mouse ileal crypts, while stimulation of α1-and β-adrenoceptors had no effect. The ef...

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Bibliographic Details
Published in:Journal of pharmacological sciences 2022-03, Vol.148 (3), p.337-341
Main Authors: Kimyo, Tomoko, Machida, Takuji, Iizuka, Kenji, Minami, Masaru, Hirafuji, Masahiko
Format: Article
Language:English
Subjects:
Ileal crypt
Intracellular calcium concentration
Noradrenaline
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Summary:The stimulation of α2-adrenoceptors caused a transient increase of intracellular calcium concentration ([Ca2+]i) monitored by ratiometry using Fura-2 in epithelial cells including enterochromaffin cells in isolated mouse ileal crypts, while stimulation of α1-and β-adrenoceptors had no effect. The effect of noradrenaline was suppressed by α2-adrenoceptor antagonists, but not by α1-and β-adrenoceptor antagonists, and partially suppressed by Ni2+ and nicardipine, but not by ω-conotoxin and ω-agatoxin. These results suggest that noradrenaline causes an increase of [Ca2+]i by the influx of extracellular Ca2+ through certain Ca2+ channels via α2-adrenoceptors in epithelial cells of mouse ileal crypts.
ISSN:1347-8613
1347-8648
DOI:10.1016/j.jphs.2022.01.010