Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation

• Published in: Frontiers in microbiology 2021-01, Vol.11, p.598507
• Main Authors: Massot, Francisco, Gkorezis, Panagiotis, Van Hamme, Jonathan, Marino, Damian, Trifunovic, Bojana Spirovic, Vukovic, Gorica, d'Haen, Jan, Pintelon, Isabel, Giulietti, Ana María, Merini, Luciano, Vangronsveld, Jaco, Thijs, Sofie
• Format: Article
• Language: English
• Subjects: EPSP synthase; glyphosate; glyphosate degradation; glyphosate tolerance; microbe-assisted phytoremediation; Microbiology; microcosm
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2020.598507

The large-scale use of the herbicide glyphosate leads to growing ecotoxicological and human health concerns. Microbe-assisted phytoremediation arises as a good option to remove, contain, or degrade glyphosate from soils and waterbodies, and thus avoid further spreading to non-target areas. To achieve this, availability of plant-colonizing, glyphosate-tolerant and -degrading strains is required and at the same time, it must be linked to plant-microorganism interaction studies focusing on a substantive ability to colonize the roots and degrade or transform the herbicide. In this work, we isolated bacteria from a chronically glyphosate-exposed site in Argentina, evaluated their glyphosate tolerance using the minimum inhibitory concentration assay, their degradation potential, their plant growth-promotion traits, and performed whole genome sequencing to gain insight into the application of a phytoremediation strategy to remediate glyphosate contaminated agronomic soils. Twenty-four soil and root-associated bacterial strains were isolated. Sixteen could grow using glyphosate as the sole source of phosphorous. As shown in MIC assay, some strains tolerated up to 10000 mg kg of glyphosate. Most of them also demonstrated a diverse spectrum of plant growth-promotion traits, confirmed in their genome sequences. Two representative isolates were studied for their root colonization. An isolate of exhibited different colonization patterns in the rhizoplane compared to an isolate of sp. Both strains were able to metabolize almost 50% of the original glyphosate concentration of 50 mg l in 9 days. In a microcosms experiment with L, performed better than , with 97% of glyphosate transformed after 20 days. The results suggest that in combination with to can be adopted for phytoremediation of glyphosate on agricultural soils. An effective strategy is presented of linking the experimental data from the isolation of tolerant bacteria with performing plant-bacteria interaction tests to demonstrate positive effects on the removal of glyphosate from soils.