Biochemical and Molecular Evaluation of Rhizobium spp. and its Growth Promotion Studies with Lentil (Lens culinaris Medik. L.)

Growth promoting Rhizobium spp are frequently used as biofertilizers for agricultural cropping system. Furthermore, Isolation, screening and biochemical characterization of Rhizobium for a specific plant is necessary to examine ability of isolated bacteria to affect the growth and development of hos...

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Bibliographic Details
Published in:Journal of pure & applied microbiology : an international research journal of microbiology 2023-03, Vol.17 (1), p.155-166
Main Authors: Banjare, Umakant, Patel, Arun Kumar, Pandey, Ashish Kumar, Kumar, Santosh, Singh, Ramesh Kumar, Masurkar, Prahlad, Singh, Rakesh Kumar, Gupta, Sandeep Kumar
Format: Article
Language:English
Subjects:
16srrna sequencing
Agricultural chemicals
Analysis
Bacteria
Beans
Cropping systems
Germination
Growth
Legumes
lentil
Mimosaceae
pgpr
rhizobium spp
RNA
seed germination
vigour index
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Summary:Growth promoting Rhizobium spp are frequently used as biofertilizers for agricultural cropping system. Furthermore, Isolation, screening and biochemical characterization of Rhizobium for a specific plant is necessary to examine ability of isolated bacteria to affect the growth and development of host plant in various ways. The current study was aimed to isolate plant specific rhizobacterial strains which are compatible with lentil (Lens culinaris Medik.L.) plant. 20 bacterial isolates have been isolated from root nodules of lentil from various agro ecological area and their biochemical characterization was performed by different plant growth promotion activities. The result showed that, among 20 isolates, four isolates have vigorous plant growth promoting activities. Four bacterial strains were able to solubilise phosphorous along with hormone production. Moreover, among four bacterial strains, two strongly produced HCN and siderophore in vitro. Subsequently, all selected bacterial isolates were inoculated in lentil seeds of variety HUL57 to study germination percentage and vigour index of the crop. Out of four isolates 26N isolate performed best growth promotion activities on lentil seedlings. Finally, on the basis of performance of bacteria on plant, four isolates were characterized using molecular approach of species identification such as 16S rRNA sequencing.
ISSN:0973-7510
2581-690X
DOI:10.22207/JPAM.17.1.04