Anti-inflammatory components of the starfish Astropecten polyacanthus

• Published in: Marine drugs 2013-08, Vol.11 (8), p.2917-2926
• Main Authors: Thao, Nguyen Phuong, Cuong, Nguyen Xuan, Luyen, Bui Thi Thuy, Quang, Tran Hong, Hanh, Tran Thi Hong, Kim, Sohyun, Koh, Young-Sang, Nam, Nguyen Hoai, Van Kiem, Phan, Van Minh, Chau, Kim, Young Ho
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Astropecten polyacanthus; Bone Marrow Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - metabolism; IL-12 p40; IL-6; Inflammation - drug therapy; Inflammation - pathology; Inhibitory Concentration 50; Interleukin-12 - metabolism; Interleukin-6 - metabolism; Lipopolysaccharides - toxicity; LPS-stimulated BMDCs; Mice; Mice, Inbred C57BL; starfish; Starfish - chemistry; TNF-α; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md11082917

Inflammation is important in biomedical research, because it plays a key role in inflammatory diseases including rheumatoid arthritis and other forms of arthritis, diabetes, heart disease, irritable bowel syndrome, Alzheimer's disease, Parkinson's disease, allergies, asthma, and even cancer. In the present study, we describe the inhibitory effect of crude extracts and steroids isolated from the starfish Astropecten polyacanthus on pro-inflammatory cytokine (Interleukin-12 (IL-12) p40, interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α)) production in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs). Among those tested, compounds 5 and 7 showed potent inhibitory effects on the production of all three pro-inflammatory cytokines with IC50 values ranging from 1.82 ± 0.11 to 7.00 ± 0.16 μM. Potent inhibitory activities were also observed for compound 1 on the production of IL-12 p40 and IL-6 with values of 3.96 ± 0.12 and 4.07 ± 0.13 μM, respectively, and for compounds 3 and 4 on the production of IL-12 p40 with values of 6.55 ± 0.18 and 5.06 ± 0.16 μM, respectively. Moreover, compounds 2 (IC50 = 34.86 ± 0.31 μM) and 6 (IC50 = 79.05 ± 2.05 μM) exhibited moderate inhibitory effects on the production of IL-12 p40, whereas compounds 3 (IC50 = 22.80 ± 0.21 μM) and 4 (IC50 = 16.73 ± 0.25 μM) moderately inhibited the production of TNF-α and IL-6, respectively.