Time-course whole blood transcriptome profiling provides new insights into Microtus fortis natural resistance mechanism to Schistosoma japonicum

Microtus fortis is known as a non-susceptible animal host of S. japonicum. A better understanding of this animal immune defense mechanism during the early stage of infection may offer an alternative route for vaccine development or therapy. Here, we analyzed the whole blood transcriptome of M. forti...

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Bibliographic Details
Published in:Heliyon 2024-10, Vol.10 (19), p.e38067, Article e38067
Main Authors: Dibo, Nouhoum, Zhou, Zhijun, Liu, Xianshu, Li, Zhuolin, Zhong, Shukun, Liu, Yan, Duan, Juan, Xia, Meng, Ma, Zhenrong, Wu, Xiang, Huang, Shuaiqin
Format: Article
Language:English
Subjects:
animals
blood
cell differentiation
gene expression regulation
immune response
Microtus
Microtus fortis
Natural resistance
Schistosoma japonicum
therapeutics
Time-course transcriptome profiling
transcriptome
unigenes
vaccine development
Whole blood
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Summary:Microtus fortis is known as a non-susceptible animal host of S. japonicum. A better understanding of this animal immune defense mechanism during the early stage of infection may offer an alternative route for vaccine development or therapy. Here, we analyzed the whole blood transcriptome of M. fortis using next-generation sequencing (NGS) to identify immune genes of biological relevance that might be involved in the mechanism of its resistance. The blood samples were collected from uninfected animals (control group) and infected animals at different time points (3, 7, 10 and 14 days post-infection). We identified 5310 sequences as unigenes and successfully annotated 4636 of them. The immune response was more intense at 10 dpi. The upregulated genes at this time point were mainly activated in the TNF and NF-kappa B signaling pathways, Th1, Th2and Th17 cell differentiation as well as cytokine-cytokine receptor interaction. Based on the differentially expressed genes analysis, we report that the IF27L2B, RETN, PGRP, IFI35, TYROBP, S100A8, S100A11, CD162, CD88, CYBA, and LBP could play important roles in the mechanism of M. fortis resistance. •The whole blood transcriptomic is suitable to elucidate the mechanism of M. fortis resistance.•IF27L2B RETN, IFI35, TYROBP, CD162, S100A11and CD88 could play important role in the mechanism of M. fortis resistance.•S. japonicum induces an inflammatory response during the early stage of infection in M. fortis.
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2024.e38067