A living thick nanofibrous implant bifunctionalized with active growth factor and stem cells for bone regeneration

• Published in: International journal of nanomedicine 2015-01, Vol.10 (default), p.1061-1075
• Main Authors: Eap, Sandy, Keller, Laetitia, Schiavi, Jessica, Huck, Olivier, Jacomine, Leandro, Fioretti, Florence, Gauthier, Christian, Sebastian, Victor, Schwinté, Pascale, Benkirane-Jessel, Nadia
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Bioengineering; Biomechanics; Biotechnology; BMP-7; Bone Morphogenetic Protein 2 - chemistry; Bone Morphogenetic Protein 2 - pharmacology; Bone morphogenetic proteins; Bone Regeneration - drug effects; Bone Substitutes - chemistry; Bone Substitutes - pharmacology; Chitosan - chemistry; Chitosan - pharmacology; electrospun nanofibers implant; Engineering Sciences; Health aspects; Human health and pathology; Humans; Life Sciences; Mechanics; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - drug effects; Mice; nanocontainers of growth factors; Nanofibers - chemistry; Nanoparticles; Original Research; Physiological aspects; Prostheses and Implants; Regeneration (Biology); regenerative nanomedicine; Rhumatology and musculoskeletal system; Skull - injuries; Stem cells
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S72670

New-generation implants focus on robust, durable, and rapid tissue regeneration to shorten recovery times and decrease risks of postoperative complications for patients. Herein, we describe a new-generation thick nanofibrous implant functionalized with active containers of growth factors and stem cells for regenerative nanomedicine. A thick electrospun poly(ε-caprolactone) nanofibrous implant (from 700 μm to 1 cm thick) was functionalized with chitosan and bone morphogenetic protein BMP-7 as growth factor using layer-by-layer technology, producing fish scale-like chitosan/BMP-7 nanoreservoirs. This extracellular matrix-mimicking scaffold enabled in vitro colonization and bone regeneration by human primary osteoblasts, as shown by expression of osteocalcin, osteopontin, and bone sialoprotein (BSPII), 21 days after seeding. In vivo implantation in mouse calvaria defects showed significantly more newly mineralized extracellular matrix in the functionalized implant compared to a bare scaffold after 30 days' implantation, as shown by histological scanning electron microscopy/energy dispersive X-ray microscopy study and calcein injection. We have as well bifunctionalized our BMP-7 therapeutic implant by adding human mesenchymal stem cells (hMSCs). The activity of this BMP-7-functionalized implant was again further enhanced by the addition of hMSCs to the implant (living materials), in vivo, as demonstrated by the analysis of new bone formation and calcification after 30 days' implantation in mice with calvaria defects. Therefore, implants functionalized with BMP-7 nanocontainers associated with hMSCs can act as an accelerator of in vivo bone mineralization and regeneration.