Seminal pro-inflammatory cytokines and pH are affected by Chlamydia infection in asymptomatic patients with teratozoospermia

• Published in: Central-European journal of immunology 2021-01, Vol.46 (1), p.76-81
• Main Authors: Pérez-Soto, Elvia, Oros-Pantoja, Rigoberto, Fernández-Martínez, Eduardo, Carbonell-Campos, Juan Manuel, Sánchez Monroy, Virginia
• Format: Article
• Language: English
• Subjects: Asymptomatic; Chlamydia; chlamydia trachomatis; Clinical Immunology; Cytokines; Enzyme-linked immunosorbent assay; Infections; Infertility; Inflammation; Interferon; Interleukin 1; Interleukin 10; Interleukin 4; Interleukin 6; Interleukin 8; Leukocytes; male infertility; pH effects; Polymerase chain reaction; Population studies; Reproductive system; Risk factors; Semen; teratozoospermia; Tumor necrosis factor; Tumor necrosis factor-TNF
• ISSN: 1426-3912; 1644-4124
• DOI: 10.5114/ceji.2021.105247

Infection and inflammation of the reproductive tract by Chlamydia trachomatis (CT) are recognized as significant risk factors for male infertility. This study aimed to evaluate CT infection and its effects on seminal parameters and cytokines in asymptomatic patients with teratozoospermia. Semen samples from one hundred four male patients were collected, and CT detection was performed by polymerase chain reaction (PCR). The quality (volume, sperm concentration, pH, motility, morphology, and leucocytes) of the semen was measured by standard procedures recommended by the World Health Organization (WHO). Pro-inflammatory cytokines [interleukin (IL)-1 , IL-6, IL-8, tumor necrosis factor (TNF- ), and interferon (IFN- )], as well as anti-inflammatory cytokines (IL-4, IL-10), were determined by using enzyme-linked immunosorbent assay (ELISA). The frequency of CT infection was expressed as a percentage. Descriptive statistics were used for comparison of cytokines from infertile men, and then the Mann-Whitney U test was applied for the contrast of seminal parameters and cytokines from CT-infected versus non-CT infected men. A ratio of 33/104 (31.7%) patients were positive for CT infection. The ejaculate of positive CT infection was found to have increased pH (pH = 7.65 in non-CT infected vs. 7.94 CT-infected men; p = 0.026). High levels of pro-inflammatory cytokines were found in the population studied; however, infected males were noted to have high levels of IL-1 [184.66 (0-3985.33 pg/ml), p = 0.001] and IL-6 [87.8 (0-1042.8 pg/ml), p = 0.001]. CT infection increased seminal pH, as well as IL-1 and IL-6 cytokines, suggesting a potential role of infection and inflammation in asymptomatic patients with teratozoospermia.