Establishment of a novel mouse model of adenomyosis suitable for longitudinal and quantitative analysis and perinatal outcome studies

The purpose of this study was to establish a novel mouse model of adenomyosis suitable for longitudinal and quantitative analyses and perinatal outcome studies. Using a 30 G needle, the entire uterine wall of one horn was mechanically punctured at a frequency of 100 times/1 cm (adenomyosis horn). Th...

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Bibliographic Details
Published in:Scientific reports 2022-10, Vol.12 (1), p.17515-11, Article 17515
Main Authors: Elsherbini, Mohammed, Koga, Kaori, Hiraoka, Takehiro, Kumasawa, Keiichi, Maki, Eiko, Satake, Erina, Taguchi, Ayumi, Makabe, Tomoko, Takeuchi, Arisa, Izumi, Gentaro, Takamura, Masashi, Harada, Miyuki, Hirata, Tetsuya, Hirota, Yasushi, Wada-Hiraike, Osamu, Osuga, Yutaka
Format: Article
Language:English
Subjects:
631/1647/767/1424
692/308
692/308/1426
692/699
692/699/2743/2730
Adenomyosis - pathology
Animals
Antigens
Blood vessels
Cell proliferation
Cytokeratin
Disease Models, Animal
Endometrium
Female
Fibrosis
Gynecology
Humanities and Social Sciences
Humans
Implantation
Ki-67 Antigen
Lesions
Litter size
Longitudinal studies
Menstruation
Mice
multidisciplinary
Obstetrics
Outcome Assessment, Health Care
Paraffin
Pathogenesis
Postpartum
Pregnancy
Quantitative analysis
Science
Science (multidisciplinary)
Smooth muscle
Uterus
Uterus - pathology
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Summary:The purpose of this study was to establish a novel mouse model of adenomyosis suitable for longitudinal and quantitative analyses and perinatal outcome studies. Using a 30 G needle, the entire uterine wall of one horn was mechanically punctured at a frequency of 100 times/1 cm (adenomyosis horn). The other horn was left unpunctured (control horn). Balb/c mice were sacrificed on day 14 (D14) or day 65 (D65) (n = 3 each). The uterus was fixed, paraffin-embedded, sliced, and stained. Lesions were detected and counted, and their volumes were measured. Cell proliferation and fibrosis were assessed by Ki67 and Masson’s Trichrome staining, respectively. Blood vessels were detected using CD31 immunostaining. Some of the mice (n = 4), were mated and the date of delivery, litter size, number of implantations, and number and volume of postpartum lesions were measured. The number of lesions per horn did not differ between D14 and D65. The volume of the entire lesion was significantly greater on D65 than on D14 (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-22413-8