Transcriptomic Analysis Reveals Dynamic Changes in Glutathione and Ascorbic Acid Content in Mango Pulp across Growth and Development Stages

• Published in: Horticulturae 2024-07, Vol.10 (7), p.694
• Main Authors: Tahir, Hassam, Sajjad, Muhammad, Qian, Minjie, Zeeshan Ul Haq, Muhammad, Tahir, Ashar, Chen, Tiantian, Shaopu, Shi, Farooq, Muhammad Aamir, Ling, Wei, Zhou, Kaibing
• Format: Article
• Language: English
• Subjects: Acidity; Acids; Antioxidants; Ascorbate oxidase; Ascorbic acid; Biotechnology; Developmental stages; Electric properties; Enzymes; Flowering; fruit ripening and senescence; Fruits; Gene regulation; Genes; Glucose 6 phosphate dehydrogenase; Glucosephosphate dehydrogenase; Glutathione; Glutathione peroxidase; Growth; Horticultural crops; L-Ascorbate peroxidase; Lipid peroxidation; mango; Mangoes; Metabolic pathways; Molecular modelling; Oxidases; Oxidative stress; Oxygen enrichment; Peroxidase; Physicochemical properties; Physiology; Pulp; Reactive oxygen species; Ripening; Scavenging; Senescence; Thiols; transcriptomic analysis; Transcriptomics; Vitamin C; γ-Glutamyltransferase
• ISSN: 2311-7524; 2311-7524
• DOI: 10.3390/horticulturae10070694

Mango (Mangifera indica) is a highly valuable horticultural crop known for its quality and productivity. This study investigates the dynamic changes in physicochemical properties and glutathione and ascorbic acid metabolic pathways in mango pulp across various growth and development stages over two consecutive years (2021–2022 and 2022–2023) by transcriptomic analysis. Overall, the results demonstrate that during different ripening periods, the pulp shows increased levels of total soluble solids, relative conductivity, glutathione, and enzymes, while titratable acidity, malondialdehyde, reactive oxygen species, and ascorbic acid contents decreased. Moreover, transcriptomic analysis identified key differentially expressed genes from the glutathione and ascorbic acid metabolic pathways and validated them with qRT-PCR. In different comparisons, a total of 1776, 2513, and 828 DEGs were identified in 30 vs. 60, 30 vs. 90, and 60 vs. 90 days after flowering, respectively. Among them, seven DEGs were primarily enriched in relevant pathways, which included ascorbate peroxidase, ascorbate oxidase, glutathione peroxidase, gamma-glutamyl transferase, glutathione transferases, and glucose-6-phosphate dehydrogenase. The upregulation of these genes indicates that glutathione and AsA respond well to scavenging reactive oxygen species and maintain normal functioning in plants. This research sheds light on the molecular mechanisms of glutathione and ascorbic acid dynamic changes in mango pulp, providing valuable insights into the regulation of antioxidant and metabolic pathways during fruit growth and development.