The Effect of Local Platelet Rich Plasma Therapy on the Composition of Bacterial Flora in Chronic Venous Leg Ulcer

• Published in: Polish journal of microbiology 2016-01, Vol.65 (3), p.353-357
• Main Authors: Woźniak, Witold, Tarnas, Monika, Miłek, Tomasz, Mlosek, Krzysztof R, Ciostek, Piotr
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Analysis; antibacterial activity; Antibacterial agents; Antibiotics; Automation; Bacteria; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacteria - isolation & purification; Blood platelets; Colonization; E coli; Female; Flora; Fungi; Fungi - classification; Fungi - drug effects; Fungi - genetics; Fungi - isolation & purification; Gram-negative bacteria; Gram-positive bacteria; Humans; Leg ulcer; Leg Ulcer - drug therapy; Leg Ulcer - microbiology; Leg ulcers; Male; Microbiota (Symbiotic organisms); microflora of venous leg ulcer; Microorganisms; Middle Aged; Plasma; platelet-rich plasma (PRP); Platelet-Rich Plasma - chemistry; Platelets; Qualitative analysis; Staphylococcal infections; Staphylococcus aureus; Staphylococcus infections; Synergism; Therapy; Ulcers; Varicose Ulcer - drug therapy; Varicose Ulcer - microbiology; venous leg ulcer
• ISSN: 1733-1331; 2544-4646; 2544-4646
• DOI: 10.5604/17331331.1215614

Microbial colonisation of chronic venous ulcers and synergism between bacterial species slow down the healing process. The study aimed at performing qualitative analysis of microbial flora in venous leg ulcers treated with platelet rich plasma (PRP). Twenty two women and twelve men aged 47-90 years were treated with PRP at our department between 2012 and 2015. Ulcer cultures collected before and after PRP therapy yielded 83 and 110 microbial isolates, respectively, of Gram positive, Gram negative bacteria and candida. Pseudomonas aueruginosa and Staphylococcus aureus were the most common pre- and post-treatment isolates. PRP therapy and increased the variety of microbial flora.