Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Antimicrobial Activity against E. coli ATCC 11775, S. maltophilia ATCC 13636 and S. enteritidis ATCC 13076

Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B-containing non-natural amino acids and the RWQWR motif were synthesized, purified, and characterized using RP-HPLC, MALDI-TOF mass spectrometry, and circular dichroism. The antibacterial activity of peptides against ATCC 1...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2017-03, Vol.22 (3), p.452
Main Authors: Huertas Méndez, Nataly De Jesús, Vargas Casanova, Yerly, Gómez Chimbi, Anyelith Katherine, Hernández, Edith, Leal Castro, Aura Lucia, Melo Diaz, Javier Mauricio, Rivera Monroy, Zuly Jenny, García Castañeda, Javier Eduardo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Anti-Bacterial Agents - chemical synthesis
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Antibacterial agents
Antimicrobial activity
Cattle
Circular Dichroism
Dichroism
E coli
Eggs
Escherichia coli
Escherichia coli - drug effects
High-performance liquid chromatography
Lactoferricin B
Lactoferrin - chemistry
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Microbial Sensitivity Tests
Minimum inhibitory concentration
Molecular Structure
Peptides
Peptides - chemical synthesis
Peptides - pharmacology
Poultry
S. enteritidis
S. maltophilia
Salmonella
Salmonella enteritidis - drug effects
Synthetic peptides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B-containing non-natural amino acids and the RWQWR motif were synthesized, purified, and characterized using RP-HPLC, MALDI-TOF mass spectrometry, and circular dichroism. The antibacterial activity of peptides against ATCC 11775, ATCC 13636, and ATCC 13076 was evaluated. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The synthetic bovine lactoferricin exhibited antibacterial activity against ATCC 11775 and ATCC 13076. The dimeric peptide (RRWQWR)₂K-Ahx exhibited the highest antibacterial activity against the tested bacterial strain. The monomeric, cyclic, tetrameric, and palindromic peptides containing the RWQWR motif exhibited high and specific activity against ATCC 11775. The results suggest that short peptides derived from lactoferricin B could be considered as potential candidates for the development of antibacterial agents against infections caused by .
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules22030452