Functional Characterization of a Syntaxin Involved in Tomato ( Solanum lycopersicum ) Resistance against Powdery Mildew

Specific syntaxins, such as Arabidopsis AtPEN1 and its barley ortholog ROR2, play a major role in plant defense against powdery mildews. Indeed, the impairment of these genes results in increased fungal penetration in both host and non-host interactions. In this study, a genome-wide survey allowed t...

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Published in:Frontiers in plant science 2017-09, Vol.8, p.1573-1573
Main Authors: Bracuto, Valentina, Appiano, Michela, Zheng, Zheng, Wolters, Anne-Marie A, Yan, Zhe, Ricciardi, Luigi, Visser, Richard G F, Pavan, Stefano, Bai, Yuling
Format: Article
Language:English
Subjects:
Blumeria graminis f. sp. hordei
Mlo resistance
Non-host resistance
Oidium neolycopersici
Plant Science
Tomato syntaxins
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Summary:Specific syntaxins, such as Arabidopsis AtPEN1 and its barley ortholog ROR2, play a major role in plant defense against powdery mildews. Indeed, the impairment of these genes results in increased fungal penetration in both host and non-host interactions. In this study, a genome-wide survey allowed the identification of 21 tomato syntaxins. Two of them, named and , are closely related to . RNAi-based silencing of in a tomato line carrying a loss-of-function mutation of the susceptibility gene led to compromised resistance toward the tomato powdery mildew fungus . Moreover, it resulted in a significant increase in the penetration rate of the non-adapted powdery mildew fungus f. sp. . Codon-based evolutionary analysis and multiple alignments allowed the detection of amino acid residues that are under purifying selection and are specifically conserved in syntaxins involved in plant-powdery mildew interactions. Our findings provide both insights on the evolution of syntaxins and information about their function which is of interest for future studies on plant-pathogen interactions and tomato breeding.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2017.01573