Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration

Background Skeletal muscle injury is one of the most common sports injuries; if not properly treated or not effective rehabilitation treatment after injury, it can be transformed into chronic cumulative injury. Curcumin, an herbal ingredient, has been found to promote skeletal muscle injury repair a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cachexia, sarcopenia and muscle sarcopenia and muscle, 2024-10, Vol.15 (5), p.1834-1849
Main Authors: Wang, Mao‐yuan, Yang, Jia‐ming, Wu, Yi, Li, Hai, Zhong, Yan‐biao, Luo, Yun, Xie, Rui‐lian
Format: Article
Language:English
Subjects:
Ca2
Cartilage
Cell culture
Cell growth
Curcumin
Growth factors
Muscle regeneration
Musculoskeletal system
Myoblast differentiation
Original
Proteins
Sports injuries
Stem cells
Wnt5a
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 1849
container_issue 5
container_start_page 1834
container_title Journal of cachexia, sarcopenia and muscle
container_volume 15
creator Wang, Mao‐yuan
Yang, Jia‐ming
Wu, Yi
Li, Hai
Zhong, Yan‐biao
Luo, Yun
Xie, Rui‐lian
description Background Skeletal muscle injury is one of the most common sports injuries; if not properly treated or not effective rehabilitation treatment after injury, it can be transformed into chronic cumulative injury. Curcumin, an herbal ingredient, has been found to promote skeletal muscle injury repair and regeneration. The Wnt5a pathway is related to the expression of myogenic regulatory factors, and Ca2+ promotes the differentiation and fusion process of myoblasts. This study explored the effect and mechanism of curcumin on myoblast differentiation during the repair and regeneration of injured skeletal muscle and its relationship with the Wnt5a pathway and Ca2+ channel. Methods Myogenic differentiation of C2C12 cells was induced with 2% horse serum, and a mouse (male, 10 weeks old) model of acute skeletal muscle injury was established using cardiotoxin (20 μL). In addition, we constructed a Wnt5a knockdown C2C12 cell model and a Wnt5a knockout mouse model. Besides, curcumin was added to the cell culture solution (80 mg/L) and fed to the mice (50 mg/kg). Fluorescence microscopy was used to determine the concentration of Ca2+. Western blot and RT‐qPCR were used to detect the protein and mRNA levels of Wnt5a, CaN, NFAT2, MyoD, Myf5, Pax7, and Myogenin. The expression levels of MyoD, Myf5, Myogenin, MHC, Desmin, and NFAT2 were detected using immunofluorescence techniques. In addition, MyoD expression was observed using immunohistochemistry, and morphological changes in mouse muscle tissue were observed using HE staining. Results During myoblast differentiation and muscle regeneration, Wnt5a expression was upregulated (P 
doi_str_mv 10.1002/jcsm.13535
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_38096579089f47e1961aa158de00e0be</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_38096579089f47e1961aa158de00e0be</doaj_id><sourcerecordid>3112224075</sourcerecordid><originalsourceid>FETCH-LOGICAL-d3465-2677e32738de113998a50a670d510d364baaeee7e79250086f4ea3985ffbe24c3</originalsourceid><addsrcrecordid>eNpdks9u1DAQxiMEolXphSewxAUJbfGf2I5PCK2AFhVxAMTRmiSTXS-JvdhOq71x6APwjDwJ7u4Kqfji0fjzb8bjr6qeM3rBKOWvN12aLpiQQj6qTjkzdKEoNY-PsTSGnVTnKW1oWbViStKn1YloTMMbo06ru-Ucu3ly_s-v39BldwMZe_LdZwlkC3l9CzsyYe9KOpEl8FekW4P3OJKwRe_8iuRAYBiwy2TahXaElEnvSiKiz-WaC56A70n6gSNmGMk0p25EEnGFHuNe8Kx6MsCY8Py4n1Xf3r_7urxcXH_-cLV8e73oRa3kgiutUXAtmh4ZE8Y0ICkoTXvJaC9U3QIgokZtuKS0UUONIEwjh6FFXnfirLo6cPsAG7uNboK4swGc3SdCXFmI2ZX2rGioUVIb2pih1siMYgBMlsqUIm2xsN4cWNu5LQPqymsjjA-gD0-8W9tVuLGM1bXSzBTCyyMhhp8zpmwnlzocR_AY5mQF1bp8n25Ykb74T7oJc_RlVlYwxjmvqZZFxQ6qWzfi7l8rjNp7p9h7p9i9U-zH5ZdP-0j8BRxutBU</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3112224075</pqid></control><display><type>article</type><title>Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration</title><source>Access via ProQuest (Open Access)</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><creator>Wang, Mao‐yuan ; Yang, Jia‐ming ; Wu, Yi ; Li, Hai ; Zhong, Yan‐biao ; Luo, Yun ; Xie, Rui‐lian</creator><creatorcontrib>Wang, Mao‐yuan ; Yang, Jia‐ming ; Wu, Yi ; Li, Hai ; Zhong, Yan‐biao ; Luo, Yun ; Xie, Rui‐lian</creatorcontrib><description><![CDATA[Background Skeletal muscle injury is one of the most common sports injuries; if not properly treated or not effective rehabilitation treatment after injury, it can be transformed into chronic cumulative injury. Curcumin, an herbal ingredient, has been found to promote skeletal muscle injury repair and regeneration. The Wnt5a pathway is related to the expression of myogenic regulatory factors, and Ca2+ promotes the differentiation and fusion process of myoblasts. This study explored the effect and mechanism of curcumin on myoblast differentiation during the repair and regeneration of injured skeletal muscle and its relationship with the Wnt5a pathway and Ca2+ channel. Methods Myogenic differentiation of C2C12 cells was induced with 2% horse serum, and a mouse (male, 10 weeks old) model of acute skeletal muscle injury was established using cardiotoxin (20 μL). In addition, we constructed a Wnt5a knockdown C2C12 cell model and a Wnt5a knockout mouse model. Besides, curcumin was added to the cell culture solution (80 mg/L) and fed to the mice (50 mg/kg). Fluorescence microscopy was used to determine the concentration of Ca2+. Western blot and RT‐qPCR were used to detect the protein and mRNA levels of Wnt5a, CaN, NFAT2, MyoD, Myf5, Pax7, and Myogenin. The expression levels of MyoD, Myf5, Myogenin, MHC, Desmin, and NFAT2 were detected using immunofluorescence techniques. In addition, MyoD expression was observed using immunohistochemistry, and morphological changes in mouse muscle tissue were observed using HE staining. Results During myoblast differentiation and muscle regeneration, Wnt5a expression was upregulated (P < 0.001) and the Wnt5a signalling pathway was activated. Wnt5a overexpression promoted the expression of MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.05), and conversely, knockdown of Wnt5a inhibited their expression (P < 0.001). The Wnt5a pathway mediated the opening of Ca2+ channels, regulated the expression levels of CaN, NFAT2, MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.01) and promoted the differentiation of C2C12 myoblasts and the repair and regeneration of injured skeletal muscle. The expression of Wnt5a, CaN, NFAT2, MyoD, Myogenin, Myf5, and MHC in C2C12 myoblast was significantly increased after curcumin intervention (P < 0.05); however, their expression decreased significantly after knocking down Wnt5a on the basis of curcumin intervention (P < 0.05). Similarly, in Wnt5a knockout mice, the promotion of muscle regeneration by curcumin was significantly attenuated. Conclusions Curcumin can activate the Wnt5a signalling pathway and mediate the opening of Ca2+ channels to accelerate the myogenic differentiation of C2C12 cells and the repair and regeneration of injured skeletal muscle.]]></description><identifier>ISSN: 2190-5991</identifier><identifier>ISSN: 2190-6009</identifier><identifier>EISSN: 2190-6009</identifier><identifier>DOI: 10.1002/jcsm.13535</identifier><identifier>PMID: 38982896</identifier><language>eng</language><publisher>Heidelberg: John Wiley &amp; Sons, Inc</publisher><subject>Ca2 ; Cartilage ; Cell culture ; Cell growth ; Curcumin ; Growth factors ; Muscle regeneration ; Musculoskeletal system ; Myoblast differentiation ; Original ; Proteins ; Sports injuries ; Stem cells ; Wnt5a</subject><ispartof>Journal of cachexia, sarcopenia and muscle, 2024-10, Vol.15 (5), p.1834-1849</ispartof><rights>2024 The Author(s). Journal of Cachexia, Sarcopenia and Muscle published by Wiley Periodicals LLC.</rights><rights>2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0009-0008-0263-2470 ; 0000-0003-1043-0904 ; 0000-0002-9928-1898 ; 0000-0003-1952-101X ; 0000-0003-2726-6135 ; 0000-0003-1423-6164</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3112224075/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3112224075?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,11562,25753,27924,27925,37012,37013,44590,46052,46476,53791,53793,75126</link.rule.ids></links><search><creatorcontrib>Wang, Mao‐yuan</creatorcontrib><creatorcontrib>Yang, Jia‐ming</creatorcontrib><creatorcontrib>Wu, Yi</creatorcontrib><creatorcontrib>Li, Hai</creatorcontrib><creatorcontrib>Zhong, Yan‐biao</creatorcontrib><creatorcontrib>Luo, Yun</creatorcontrib><creatorcontrib>Xie, Rui‐lian</creatorcontrib><title>Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration</title><title>Journal of cachexia, sarcopenia and muscle</title><description><![CDATA[Background Skeletal muscle injury is one of the most common sports injuries; if not properly treated or not effective rehabilitation treatment after injury, it can be transformed into chronic cumulative injury. Curcumin, an herbal ingredient, has been found to promote skeletal muscle injury repair and regeneration. The Wnt5a pathway is related to the expression of myogenic regulatory factors, and Ca2+ promotes the differentiation and fusion process of myoblasts. This study explored the effect and mechanism of curcumin on myoblast differentiation during the repair and regeneration of injured skeletal muscle and its relationship with the Wnt5a pathway and Ca2+ channel. Methods Myogenic differentiation of C2C12 cells was induced with 2% horse serum, and a mouse (male, 10 weeks old) model of acute skeletal muscle injury was established using cardiotoxin (20 μL). In addition, we constructed a Wnt5a knockdown C2C12 cell model and a Wnt5a knockout mouse model. Besides, curcumin was added to the cell culture solution (80 mg/L) and fed to the mice (50 mg/kg). Fluorescence microscopy was used to determine the concentration of Ca2+. Western blot and RT‐qPCR were used to detect the protein and mRNA levels of Wnt5a, CaN, NFAT2, MyoD, Myf5, Pax7, and Myogenin. The expression levels of MyoD, Myf5, Myogenin, MHC, Desmin, and NFAT2 were detected using immunofluorescence techniques. In addition, MyoD expression was observed using immunohistochemistry, and morphological changes in mouse muscle tissue were observed using HE staining. Results During myoblast differentiation and muscle regeneration, Wnt5a expression was upregulated (P < 0.001) and the Wnt5a signalling pathway was activated. Wnt5a overexpression promoted the expression of MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.05), and conversely, knockdown of Wnt5a inhibited their expression (P < 0.001). The Wnt5a pathway mediated the opening of Ca2+ channels, regulated the expression levels of CaN, NFAT2, MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.01) and promoted the differentiation of C2C12 myoblasts and the repair and regeneration of injured skeletal muscle. The expression of Wnt5a, CaN, NFAT2, MyoD, Myogenin, Myf5, and MHC in C2C12 myoblast was significantly increased after curcumin intervention (P < 0.05); however, their expression decreased significantly after knocking down Wnt5a on the basis of curcumin intervention (P < 0.05). Similarly, in Wnt5a knockout mice, the promotion of muscle regeneration by curcumin was significantly attenuated. Conclusions Curcumin can activate the Wnt5a signalling pathway and mediate the opening of Ca2+ channels to accelerate the myogenic differentiation of C2C12 cells and the repair and regeneration of injured skeletal muscle.]]></description><subject>Ca2</subject><subject>Cartilage</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Curcumin</subject><subject>Growth factors</subject><subject>Muscle regeneration</subject><subject>Musculoskeletal system</subject><subject>Myoblast differentiation</subject><subject>Original</subject><subject>Proteins</subject><subject>Sports injuries</subject><subject>Stem cells</subject><subject>Wnt5a</subject><issn>2190-5991</issn><issn>2190-6009</issn><issn>2190-6009</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdks9u1DAQxiMEolXphSewxAUJbfGf2I5PCK2AFhVxAMTRmiSTXS-JvdhOq71x6APwjDwJ7u4Kqfji0fjzb8bjr6qeM3rBKOWvN12aLpiQQj6qTjkzdKEoNY-PsTSGnVTnKW1oWbViStKn1YloTMMbo06ru-Ucu3ly_s-v39BldwMZe_LdZwlkC3l9CzsyYe9KOpEl8FekW4P3OJKwRe_8iuRAYBiwy2TahXaElEnvSiKiz-WaC56A70n6gSNmGMk0p25EEnGFHuNe8Kx6MsCY8Py4n1Xf3r_7urxcXH_-cLV8e73oRa3kgiutUXAtmh4ZE8Y0ICkoTXvJaC9U3QIgokZtuKS0UUONIEwjh6FFXnfirLo6cPsAG7uNboK4swGc3SdCXFmI2ZX2rGioUVIb2pih1siMYgBMlsqUIm2xsN4cWNu5LQPqymsjjA-gD0-8W9tVuLGM1bXSzBTCyyMhhp8zpmwnlzocR_AY5mQF1bp8n25Ykb74T7oJc_RlVlYwxjmvqZZFxQ6qWzfi7l8rjNp7p9h7p9i9U-zH5ZdP-0j8BRxutBU</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Wang, Mao‐yuan</creator><creator>Yang, Jia‐ming</creator><creator>Wu, Yi</creator><creator>Li, Hai</creator><creator>Zhong, Yan‐biao</creator><creator>Luo, Yun</creator><creator>Xie, Rui‐lian</creator><general>John Wiley &amp; Sons, Inc</general><general>John Wiley and Sons Inc</general><general>Wiley</general><scope>24P</scope><scope>WIN</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0009-0008-0263-2470</orcidid><orcidid>https://orcid.org/0000-0003-1043-0904</orcidid><orcidid>https://orcid.org/0000-0002-9928-1898</orcidid><orcidid>https://orcid.org/0000-0003-1952-101X</orcidid><orcidid>https://orcid.org/0000-0003-2726-6135</orcidid><orcidid>https://orcid.org/0000-0003-1423-6164</orcidid></search><sort><creationdate>202410</creationdate><title>Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration</title><author>Wang, Mao‐yuan ; Yang, Jia‐ming ; Wu, Yi ; Li, Hai ; Zhong, Yan‐biao ; Luo, Yun ; Xie, Rui‐lian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d3465-2677e32738de113998a50a670d510d364baaeee7e79250086f4ea3985ffbe24c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Ca2</topic><topic>Cartilage</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Curcumin</topic><topic>Growth factors</topic><topic>Muscle regeneration</topic><topic>Musculoskeletal system</topic><topic>Myoblast differentiation</topic><topic>Original</topic><topic>Proteins</topic><topic>Sports injuries</topic><topic>Stem cells</topic><topic>Wnt5a</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Mao‐yuan</creatorcontrib><creatorcontrib>Yang, Jia‐ming</creatorcontrib><creatorcontrib>Wu, Yi</creatorcontrib><creatorcontrib>Li, Hai</creatorcontrib><creatorcontrib>Zhong, Yan‐biao</creatorcontrib><creatorcontrib>Luo, Yun</creatorcontrib><creatorcontrib>Xie, Rui‐lian</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley-Blackwell Free Backfiles(OpenAccess)</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Journal of cachexia, sarcopenia and muscle</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Mao‐yuan</au><au>Yang, Jia‐ming</au><au>Wu, Yi</au><au>Li, Hai</au><au>Zhong, Yan‐biao</au><au>Luo, Yun</au><au>Xie, Rui‐lian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration</atitle><jtitle>Journal of cachexia, sarcopenia and muscle</jtitle><date>2024-10</date><risdate>2024</risdate><volume>15</volume><issue>5</issue><spage>1834</spage><epage>1849</epage><pages>1834-1849</pages><issn>2190-5991</issn><issn>2190-6009</issn><eissn>2190-6009</eissn><abstract><![CDATA[Background Skeletal muscle injury is one of the most common sports injuries; if not properly treated or not effective rehabilitation treatment after injury, it can be transformed into chronic cumulative injury. Curcumin, an herbal ingredient, has been found to promote skeletal muscle injury repair and regeneration. The Wnt5a pathway is related to the expression of myogenic regulatory factors, and Ca2+ promotes the differentiation and fusion process of myoblasts. This study explored the effect and mechanism of curcumin on myoblast differentiation during the repair and regeneration of injured skeletal muscle and its relationship with the Wnt5a pathway and Ca2+ channel. Methods Myogenic differentiation of C2C12 cells was induced with 2% horse serum, and a mouse (male, 10 weeks old) model of acute skeletal muscle injury was established using cardiotoxin (20 μL). In addition, we constructed a Wnt5a knockdown C2C12 cell model and a Wnt5a knockout mouse model. Besides, curcumin was added to the cell culture solution (80 mg/L) and fed to the mice (50 mg/kg). Fluorescence microscopy was used to determine the concentration of Ca2+. Western blot and RT‐qPCR were used to detect the protein and mRNA levels of Wnt5a, CaN, NFAT2, MyoD, Myf5, Pax7, and Myogenin. The expression levels of MyoD, Myf5, Myogenin, MHC, Desmin, and NFAT2 were detected using immunofluorescence techniques. In addition, MyoD expression was observed using immunohistochemistry, and morphological changes in mouse muscle tissue were observed using HE staining. Results During myoblast differentiation and muscle regeneration, Wnt5a expression was upregulated (P < 0.001) and the Wnt5a signalling pathway was activated. Wnt5a overexpression promoted the expression of MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.05), and conversely, knockdown of Wnt5a inhibited their expression (P < 0.001). The Wnt5a pathway mediated the opening of Ca2+ channels, regulated the expression levels of CaN, NFAT2, MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.01) and promoted the differentiation of C2C12 myoblasts and the repair and regeneration of injured skeletal muscle. The expression of Wnt5a, CaN, NFAT2, MyoD, Myogenin, Myf5, and MHC in C2C12 myoblast was significantly increased after curcumin intervention (P < 0.05); however, their expression decreased significantly after knocking down Wnt5a on the basis of curcumin intervention (P < 0.05). Similarly, in Wnt5a knockout mice, the promotion of muscle regeneration by curcumin was significantly attenuated. Conclusions Curcumin can activate the Wnt5a signalling pathway and mediate the opening of Ca2+ channels to accelerate the myogenic differentiation of C2C12 cells and the repair and regeneration of injured skeletal muscle.]]></abstract><cop>Heidelberg</cop><pub>John Wiley &amp; Sons, Inc</pub><pmid>38982896</pmid><doi>10.1002/jcsm.13535</doi><tpages>16</tpages><orcidid>https://orcid.org/0009-0008-0263-2470</orcidid><orcidid>https://orcid.org/0000-0003-1043-0904</orcidid><orcidid>https://orcid.org/0000-0002-9928-1898</orcidid><orcidid>https://orcid.org/0000-0003-1952-101X</orcidid><orcidid>https://orcid.org/0000-0003-2726-6135</orcidid><orcidid>https://orcid.org/0000-0003-1423-6164</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2190-5991
ispartof Journal of cachexia, sarcopenia and muscle, 2024-10, Vol.15 (5), p.1834-1849
issn 2190-5991
2190-6009
2190-6009
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_38096579089f47e1961aa158de00e0be
source Access via ProQuest (Open Access); Wiley Online Library Open Access; PubMed Central
subjects Ca2
Cartilage
Cell culture
Cell growth
Curcumin
Growth factors
Muscle regeneration
Musculoskeletal system
Myoblast differentiation
Original
Proteins
Sports injuries
Stem cells
Wnt5a
title Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T13%3A13%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Curcumin%E2%80%90activated%20Wnt5a%20pathway%20mediates%20Ca2+%20channel%20opening%20to%20affect%20myoblast%20differentiation%20and%20skeletal%20muscle%20regeneration&rft.jtitle=Journal%20of%20cachexia,%20sarcopenia%20and%20muscle&rft.au=Wang,%20Mao%E2%80%90yuan&rft.date=2024-10&rft.volume=15&rft.issue=5&rft.spage=1834&rft.epage=1849&rft.pages=1834-1849&rft.issn=2190-5991&rft.eissn=2190-6009&rft_id=info:doi/10.1002/jcsm.13535&rft_dat=%3Cproquest_doaj_%3E3112224075%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-d3465-2677e32738de113998a50a670d510d364baaeee7e79250086f4ea3985ffbe24c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3112224075&rft_id=info:pmid/38982896&rfr_iscdi=true