Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles

OBJECTIVECarcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen family. Although its expression has been found in chronic oral inflammatory epithelium, this study aimed to know whether CEACAM1 in oral keratinocytes participates in host immune...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied oral science 2022, Vol.30, p.e20220158-e20220158
Main Authors: SAKUMA, Miyuki, OHTA, Kouji, FUKADA, Shohei, AKAGI, Misaki, KATO, Hiroki, ISHIDA, Yoko, NARUSE, Takako, TAKECHI, Masaaki, SHIGEISHI, Hideo, NISHI, Hiromi, AIKAWA, Tomonao
Format: Article
Language:English
Subjects:
Candida albicans
CEACAM1
DENTISTRY, ORAL SURGERY & MEDICINE
HO-1
Oral keratinocytes
Original
β-glucan-containing particles
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:OBJECTIVECarcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen family. Although its expression has been found in chronic oral inflammatory epithelium, this study aimed to know whether CEACAM1 in oral keratinocytes participates in host immune response against Candida albicans . METHODOLOGYWe investigated CEACAM1 expression in oral keratinocytes induced by C. albicans as well as by Candida cell wall component β-glucan particles (β-GPs). Furthermore, the effects of CEACAM1 on β-GPs-induced heme oxygenase-1 (HO-1) expression and its related signals were examined. RESULTSFluorescence staining showed CEACAM1 expression in oral keratinocytes (RT7) cells, whereas quantitative reverse transcription (RT)-PCR indicated that both live and heat-killed C. albicans increased CEACAM1 mRNA expression in RT7 cells. Examinations using quantitative RT-PCR and western blotting indicated that CEACAM1 expression was also increased by β-GPs derived from C. albicans . Specific siRNA for CEACAM1 decreased HO-1 expression induced by β-GPs from C. albicans as well as the budding yeast microorganism Saccharomyces cerevisiae . Moreover, knockdown of CEACAM1 decreased β-GPs-induced ROS activity in the early phase and translocation of Nrf2 into the nucleus. CONCLUSIONCEACAM1 in oral keratinocytes may have a critical role in regulation of HO-1 for host immune defense during Candida infection.
ISSN:1678-7757
1678-7765
1678-7765
DOI:10.1590/1678-7757-2022-0158