Increased expression of the calcium-activated chloride channel hCLCA1 in airways of patients with obstructive chronic bronchitis

Interleukin (IL)-9 and its effect on enhancing the human calcium-activated chloride channel 1 (hCLCA1) expression have been shown to induce mucin production. Increased expression of hCLCA1 may, in turn, contribute to mucus overproduction in chronic obstructive pulmonary disease (COPD) with a chronic...

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Published in:Canadian respiratory journal 2005, Vol.12 (3), p.143-146
Main Authors: Hauber, Hans-Peter, Bergeron, Celine, Tsicopoulos, Anne, Wallaert, Benoit, Olivenstein, Ron, Holroyd, Kenneth J, Levitt, Roy C, Hamid, Qutayba
Format: Article
Language:English
Subjects:
Aged
Chloride Channels - metabolism
Humans
Male
Middle Aged
Periodic Acid-Schiff Reaction
Pulmonary Disease, Chronic Obstructive - metabolism
Respiratory Mucosa - metabolism
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Summary:Interleukin (IL)-9 and its effect on enhancing the human calcium-activated chloride channel 1 (hCLCA1) expression have been shown to induce mucin production. Increased expression of hCLCA1 may, in turn, contribute to mucus overproduction in chronic obstructive pulmonary disease (COPD) with a chronic bronchitis (CB) phenotype. To determine the expression of IL-9, IL-9 receptor (IL-9R), hCLCA1 and mucoglycoconjugates in COPD. Bronchial biopsies were obtained from six patients with obstructive CB and six healthy control subjects. IL-9, IL-9R and hCLCA1 expression were detected using immunohistochemistry. Additionally, in situ hybridization was performed to determine the expression of hCLCA1 messenger RNA. Mucin production was assessed using periodic acid-Schiff staining. There was a significantly higher number of IL-9 immunoreactive cells in the submucosa of patients with COPD than that of healthy control subjects (P
ISSN:1198-2241
1916-7245
DOI:10.1155/2005/531432