Generation of Functional Mouse Hippocampal Neurons

Primary culture of mouse hippocampal neurons is a very useful model for studying neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. Here we describe a step-by-step process of generating primary neurons from mouse embryonic hippocampi (E17.5/E...

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Bibliographic Details
Published in:Bio-protocol 2020-08, Vol.10 (15)
Main Authors: Tomassoni-Ardori, Francesco, Hong, Zhenyi, Fulgenzi, Gianluca, Tessarollo, Lino
Format: Article
Language:English
Subjects:
Biology
Methods
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Summary:Primary culture of mouse hippocampal neurons is a very useful model for studying neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. Here we describe a step-by-step process of generating primary neurons from mouse embryonic hippocampi (E17.5/E18.5). Hippocampal neurons generated with this protocol can be plated in different tissue culture dishes according to different experimental aims and can produce a reliable source of pure and differentiated neurons in less than one week. This protocol covers all the steps necessary for the preparation, culture and characterization of the neuronal culture, including the illustration of dissection instruments, surgical procedure for embryos' isolation, culturing conditions and assessment of culture's purity and differentiation. Evaluation of neuronal activity was performed by analysis of calcium imaging dynamics at six days in culture.
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.3702