Molecular interaction of human acetylcholinesterase with trans-tephrostachin and derivatives for Alzheimer's disease

Alzheimer's disease (AD), a neurodegenerative disorder affects more than 35 million people globally. Acetylcholinesterase suppression is the common approach to enhance the well-being of AD patients by increasing the duration of acetylcholine in the cholinergic synapses. Generally, herbal second...

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Bibliographic Details
Published in:Heliyon 2020-09, Vol.6 (9), p.e04930-e04930, Article e04930
Main Authors: Pitchai, Arjun, Rajaretinam, Rajesh Kannan, Mani, Rajasekar, Nagarajan, Nagasundaram
Format: Article
Language:English
Subjects:
Acetylcholinesterase (AChE)
Biochemistry
Enzyme inhibition kinetics
Flavonoids
Molecular docking and dynamics
Neuroscience
Pharmacology
Trans-Tephrostachin derivatives
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Summary:Alzheimer's disease (AD), a neurodegenerative disorder affects more than 35 million people globally. Acetylcholinesterase suppression is the common approach to enhance the well-being of AD patients by increasing the duration of acetylcholine in the cholinergic synapses. Generally, herbal secondary metabolites are reported to be a major resource for acetylcholinesterase inhibitors (AChEIs). Trans-tephrostachin was reported from Tephrosia purpurea for AChE inhibition. Here, we report on the design, synthesis, and assessment of human acetylcholinesterase inhibitory activity from trans-tephrostachin derivatives or analogs as anti-AD agents. The five newly synthesized compounds 4a. 4b, 4c, 4d and 4e displayed potent inhibitory activities with IC50 values of 35.0, 35.6, 10.6, 10.3, and 28.1 μM respectively. AChE enzyme kinetic study was performed for the five derived compounds using the Ellman's method. The Lineweaver-Burk and the secondary plots revealed the mixed inhibition for 4a, 4c and 4d whereas 4b and 4e demonstrated competitive inhibition. Molecular docking and molecular dynamics simulations showed the derivatives or analogs of trans-tephrostachin attained a high binding affinity and efficacy than the standard drug. In conclusion, trans-tephrostachin and its derivative compounds could become effective agents for further drug development to treat AD. Acetylcholinesterase (AChE), Flavonoids; Trans-Tephrostachin derivatives, Enzyme inhibition kinetics, Molecular docking and dynamics, Biochemistry, Neuroscience, Pharmacology
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2020.e04930