Crude extract and isolated bioactive compounds from Notholirion thomsonianum (Royale) Stapf as multitargets antidiabetic agents: in-vitro and molecular docking approaches

• Published in: BMC complementary and alternative medicine 2021-10, Vol.21 (1), p.270-270, Article 270
• Main Authors: Mahnashi, Mater H, Alqahtani, Yahya S, Alqarni, Ali O, Alyami, Bandar A, Jan, Muhammad Saeed, Ayaz, Muhammad, Ullah, Farhat, Rashid, Umer, Sadiq, Abdul
• Format: Article
• Language: English
• Subjects: Acarbose; Acetic acid; Amylases; Analysis; Antidiabetics; Antioxidant; Antioxidants; Antioxidants - pharmacology; Assaying; Binding; Bioactive compounds; Biological activity; Diabetes; Diabetes mellitus; Diabetes Mellitus - drug therapy; Diabetes therapy; Diabetic neuropathy; Drugs; Enzymes; Ethyl acetate; Experiments; Free radicals; Glucosidase; Herbal medicine; Humans; Hydrogen peroxide; Hyperglycemia; Hyperglycemia - drug therapy; Hypoglycemic agents; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - pharmacology; Insulin resistance; Medicinal plants; Medicine, Botanic; Medicine, Herbal; Metabolism; Molecular docking; Molecular Docking Simulation; Molecular Structure; Natural products; NMR; Notholirion thomsonianum; Nuclear magnetic resonance; Pakistan; Phosphatases; Plant Extracts - chemistry; Plant Extracts - pharmacology; Protein tyrosine phosphatase 1B (PTP1B); Protein-tyrosine-phosphatase; Type 2 diabetes; Tyrosine; Ursolic acid; World population; α-Amylase; α-Glucosidase
• ISSN: 2662-7671; 2662-7671; 1472-6882
• DOI: 10.1186/s12906-021-03443-7

Diabetes mellitus is a common disease effecting the lifestyles of majority world population. In this research work, we have embarked the potential role of crude extracts and isolated compounds of Notholirion thomsonianum for the management diabetes mellitus. The crude extracts of N. thomsonianum were initially evaluated for α-glucosidase, α-amylase and antioxidant activities. The compounds were isolated from the activity based potent solvent fraction. The structures of isolated compounds were confirmed with NMR and MS analyses. The isolated compounds were tested for α-glucosidase, α-amylase, protein tyrosine phosphatase 1B (PTP1B) and DPPH activities. The molecular docking studies were carried out to find the binding interactions of isolated compounds for α-glucosidase, α-amylase and PTP1B. Initially, we screened out crude extracts and subfractions of N. thomsonianum against different in-vitro targets. Among all, Nt.EtAc was observed a potent fraction among all giving IC values of 67, 70,