Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches

Recent advances in imaging technology have made it possible to track cellular recruitment and behavior within the vasculature of living animals in real-time. Using approaches such as resonant scanning confocal and multiphoton intravital microscopy (IVM), we are now able to observe cells within the i...

Full description

Saved in:
Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2018-07, Vol.7 (7), p.69
Main Authors: Turk, Madison, Naumenko, Victor, Mahoney, Douglas J, Jenne, Craig N
Format: Article
Language:English
Subjects:
Antitumor agents
Cancer
Cell interactions
Cloning
Cytotoxicity
Drug development
Flow cytometry
Gene expression
imaging
Immune system
Immunotherapy
intravital
Labeling
Laboratories
Lasers
Leukocyte migration
leukocytes
Leukocytes (neutrophilic)
Lymphocytes T
Medical research
Microscopy
Monocytes
Neutrophils
Pathogens
Physiology
Scanning
trafficking
Tumor microenvironment
Tumors
vasculature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recent advances in imaging technology have made it possible to track cellular recruitment and behavior within the vasculature of living animals in real-time. Using approaches such as resonant scanning confocal and multiphoton intravital microscopy (IVM), we are now able to observe cells within the intact tumor microenvironment of a mouse. We are able to follow these cells for extended periods of time (hours) and can characterize how specific cell types (T cells, neutrophils, monocytes) interact with the tumor vasculature and cancer cells. This approach provides greater insight into specific cellular behaviors and cell⁻cell interactions than conventional techniques such as histology and flow cytometry. In this report, we describe the surgical preparation of animals to expose the tumor and both resonant scanning confocal and multiphoton imaging approaches used to track leukocyte recruitment, adhesion, and behavior within the tumor microenvironment. We present techniques for the measurement and quantification of leukocyte behavior within the bloodstream and tumor interstitium. The use of IVM to study leukocyte behavior within the tumor microenvironment provides key information not attainable with other approaches, that will help shape the development of better, more effective anticancer drugs and therapeutic approaches.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells7070069